1887

Abstract

SUMMARY: Treatment of the cells of various spp. with an alkaline solution of sodium hypochlorite resulted in dissolution of the cells and liberation of the intracellular lipid inclusion bodies. Analyses of the isolated and purified inclusions of grown under a variety of cultural conditions showed them to contain about 89 % poly--hydroxybutyrate and 11% ether-soluble lipid. Parallel estimations of the poly- -hydroxybutyrate content of intact organisms by Lemoigne’s chloroform extraction method showed that all of it was present in the lipid inclusions. These observations form the basis of a simple and rapid method of estimating the poly--hydroxybutyrate content of spp. It consists essentially of digesting a washed bacterial suspension with a standard alkaline hypochlorite solution under standard conditions and measuring the residual turbidity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-19-1-198
1958-08-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/19/1/mic-19-1-198.html?itemId=/content/journal/micro/10.1099/00221287-19-1-198&mimeType=html&fmt=ahah

References

  1. Ashby G. K. 1938; Simplified Schaeffer spore strain. Science 87:443
    [Google Scholar]
  2. Burdon K. L. 1946; Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. J. Bact 52:665
    [Google Scholar]
  3. Cain A. J. 1950; The histochemistry of lipoids of animals. Biol. Rev 25:73
    [Google Scholar]
  4. Duguid J. P., Smith I. W., Wilkinson J. F. 1954; Volutin production in Bacterium aerogenes due to development of an acid reaction. J. Path. Bact 67:289
    [Google Scholar]
  5. Duguid J. P., Wilkinson J. F. 1953; The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. J. gen. Microbiol 9:174
    [Google Scholar]
  6. Feigl F. 1947 The Quantitative Analysis by Spot Tests, 3rd edition. p. 408 New York: Elsevier Publishing Co. Inc;
    [Google Scholar]
  7. Fiske A., SubbaRow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem 66:375
    [Google Scholar]
  8. Képès A., Péaud-Lenoël C. 1952; Sur les propriétés et la constitution des lipides β-hydroxybutyriques. C.R. Acad. Sci., Paris 234:756
    [Google Scholar]
  9. Laybourne R. L. 1924; A modification of Albert’s stain for the diphtheria bacillus. J. Amer. med. Ass 83:121
    [Google Scholar]
  10. Lemoigne M. 1927; Études sur l’autolyse microbienne. Origine de l’acide β-oxy-butyrique formé par autolyse. Ann. Inst. Pasteur 41:148
    [Google Scholar]
  11. Lemoigne M., Delaporte B., Croson M. 1944; Contribution a l’étude botanique et biochimique des bactéries du genre Bacillus. 2. Valeur du test des lipides β-hydroxybutyriques pour la characterisation des espèces. Ann. Inst. Pasteur 70:224
    [Google Scholar]
  12. Lemoigne M., Roukhelman N. 1940; Caractérisation et évolution des produits de déshydration et de polymerisation de l’acide β-hydroxybutyrique. Ann. Ferment 5:527
    [Google Scholar]
  13. Macrae R. M., Wilkinson J. F. 1958; Poly-β-hydroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium. J. gen. Microbiol 19:210
    [Google Scholar]
  14. Mazia D., Brewer P. A., Alfert M. 1953; The cytological staining and measurement of protein with mercuric bromophenol blue. Biol. Bull., Wood’s Hole 104–105:57
    [Google Scholar]
  15. Meyer A. 1901; Notiz über das Verhalten der Sporen und Fettropfen der Bakterien gegen Eau de Javelle und gegen Chloralhydratlösung. Zbl. Bakt. (1 Abt. Orig.) 29:809
    [Google Scholar]
  16. Weibull C. 1953; Characterisation of the protoplasmic constituents of Bacillus megaterium. J. Bact 66:696
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-19-1-198
Loading
/content/journal/micro/10.1099/00221287-19-1-198
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error