1887

Abstract

The effect of the iron concentration in the medium on the concentration of toxin and coproporphyrin in the culture filtrate of a strain of has been found to differ quantitatively from that previously reported in the case of the P.W. 8 strain. Only 50-60% of the iron present in the medium, at all concentrations tested, was recoverable from the organisms. Of the total iron present in the medium, only 9% was found to be present in the organisms as a haem complex capable of measurement as its pyridine haemochromogen. On the basis of their iron contents protohaemin and iron coproporphyrin III are less active than ferrous sulphate in their effect on toxin yield. These findings do not support the hypothesis that diphtheria toxin is the protein moiety of the cytochrome of the cell.

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1958-06-01
2022-09-24
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References

  1. Campbell-smith F. 1930; The nature of diphtheria toxin-antitoxin floccules. Lancet i:529
    [Google Scholar]
  2. Clarke W.A., Clarke G.D. 1953; The mechanism of the effect of iron and cobalt on the production of toxin by Corynebacterium diphtheriae. . Biochem. J 55:xvi
    [Google Scholar]
  3. Coulter C.B., Stone F.M. 1930; The occurrence of porphyrins in cultures of Corynebacterium diphtheriae. . J. gen. Physiol. 14:583
    [Google Scholar]
  4. Fischer H., Orth H. 1937 Die Chemie des Pyrrols Pyrrolfarbstoffe, II. Erste Hälfte.Leipzig: Academische Verlagsgesellschaft;
    [Google Scholar]
  5. Fortune W.B., Mellon M.G. 1938; Determination of iron with o-phen- anthroline. Industr. Engng Chem. (Anal.) 10: 60
    [Google Scholar]
  6. Gray C.H., Holt L.B. 1948; The isolation of coproporphyrin III from Corynebacterium diphtheriae culture filtrates. Biochem. J 43:191
    [Google Scholar]
  7. Holden F.H. 1941; The ultraviolet absorption spectra of some metalloporphy-rins and of their compounds with globin. . Aust. J. exp. Med. biol. Sci. 19:1
    [Google Scholar]
  8. Holt L.B. 1948; The preparation of purified and concentrated diphtheria toxoid from a semi-synthetic medium. Brit. J. exp. Path 29:385
    [Google Scholar]
  9. Lascelles J. 1956; The synthesis of porphyrins and bacterial chlorophyl by cell suspensions of Rhodopseudomonas spheroides. . Biochem. J 62:78
    [Google Scholar]
  10. Markham R. 1942; A steam-distillation apparatus suitable for micro-Kjeldahl analysis. Biochem. J 36:790
    [Google Scholar]
  11. Pappenheimer A.M. Jun 1942; Studies on diphtheria toxin and its reaction with antitoxin. J. Bact 43:273
    [Google Scholar]
  12. Pappenheimer A.M. Jun 1947; Diphtheria toxin. III. A reinvestigation of the effect of iron on toxin and porphyrin production. J. biol. Chem. 167:251
    [Google Scholar]
  13. Pappenheimer A.M. Jun Hendee E.D. 1947; Diphtheria toxin. IV. The iron enzymes of Corynebacterium diphtheriae and their possible relationship to diphtheria toxin. J. biol. Chem. 171:701
    [Google Scholar]
  14. Pappenheimer A.M. Jun Hendee E.D. 1949; Diphtheria toxin. V. A comparison between the diphtherial succinoxidase system and that of beef heart muscle. J. biol. Chem 180:597
    [Google Scholar]
  15. Pappenheimer A.M. Jun Johnson S.J. 1936; Studies in diphtheria toxin production. I. The effect of iron and copper. Brit. J. exp. Path 17:335
    [Google Scholar]
  16. Pappenheimer A.M. Jr. Williams C.M. 1952; The effect of diphtheria toxin on the Cecropia silkworm. J. gen. Physiol. 35:727
    [Google Scholar]
  17. Ramon G. 1922; A propos du titrage in vitro du serum antidiphtherique par la floculation. . C.R. Soc. Biol. Paris 86813
    [Google Scholar]
  18. Rawlinson W.A., Hale J.H. 1949; Prosthetic groups of the cytochromes present in Corynebacterium diphtheriae with especial reference to cytochrome a. . Biochem. J. 45:247
    [Google Scholar]
  19. Raynaud M., Turpin A., Mongola B., Bizzini B., Pery R. 1954; Croissance et Toxinogenese. Ann. Inst.Pasteur, 87:599
    [Google Scholar]
  20. Saywell L.G., Cunningham B.B. 1937; Determination of Iron. Colorimetric o-phenanthroline method. Industr. Engng Chem. (Anal.) 9:67
    [Google Scholar]
  21. Stern K.G. 1936; The constitution of the prosthetic group of catalase. J. biol. Chem 112:661
    [Google Scholar]
  22. Theorell H. 1940; Reversible Spaltung einer Peroxydase. Ark. Kemi Min. Geol. . 148: No.20
    [Google Scholar]
  23. Wadsworth A., Crowe M.O’L., Smith L.A. 1935; The spectroscopic investigation of bacterial toxins: the absorption spectra of the products of Corynebacterium diphtheriae. . Brit. J. exp. Path 16:201
    [Google Scholar]
  24. Wheeler M.W., Crowe M.O’L. 1936; A note on conditions affecting the production of toxin and porphyrins by the diphtheria bacillus. J. Bact 31:519
    [Google Scholar]
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