1887

Abstract

Summary: A modification of Fontana’s silver-plating method was devised and used for the eytological study of 214 isolates of spp. Many features, such as flagella, cross-walls or septa, capsules and slime were thereby clearly demonstrated simultaneously and easily. Comparisons were made with other published methods for staining flagella, capsules and slime, and good agreement was obtained. No totally non-flagellate isolate was found, nor were flagella lost during maintenance for 3–5 ½ years in the laboratory. The number of flagella/rod and also bipolar flagellation were found to be characters of little value for species differentiation. The flagellation of young cultures was not found to be significantly different from that observed in older cultures; the polar position of the flagella was confirmed. Flagella curvature (wavelength and amplitude) as seen in silver-plated organisms prepared under reasonably standardized conditions, was noted and considered to have no usefulness for subgeneric classification.

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1958-06-01
2024-04-25
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References

  1. Anthony E.E. 1931; See Manual of Methods for the Pure Culture Study of Bacteria. Leaflet IV. Staining Methods 1954, p. 18
    [Google Scholar]
  2. Bartholomew J.W. 1949; Flagellation of certain species of Pseudomonas as seen with the electron microscope. J. gen. Microbiol 3:340
    [Google Scholar]
  3. Bergey’s Manud of Determinative Bacteriology 1948, 6th ed.. Breed R. S., Murray E. G. D., Hitchens A.P. Edited by London: Bailliére, Tindall and Cox;
  4. Bisset K.A. 1951; The development of the surface structures in dividing bacteria. J. gen. Microbiol 5:155
    [Google Scholar]
  5. Bisset K.A., Hale C.M.F. 1951; The development of bacterial flagella in the germinating microcyst. J. gen. Microbiol 5:150
    [Google Scholar]
  6. Conn H.J., Elrod R.P. 1947; Concerning flagellation and motility. J. Bact 54:681
    [Google Scholar]
  7. Gray P.H.H. 1926; A method of staining bacterial flagella. J. Bact 12:273
    [Google Scholar]
  8. Houwink A.L., Iterson W.van. 1950; Electron microscopical observations on bacterial cytology. II. A study on flagellation. Biochim. Biophys.Acta 5:10
    [Google Scholar]
  9. Iterson W.van. 1947; Some electron microscopical observations on bacterial cytology. Biochim. Biophys.Acta 1:527
    [Google Scholar]
  10. Klieneberger-Nobel E. 1948; Capsules and mucoid envelopes of bacteria. J. Hyg., Carrib 46:345
    [Google Scholar]
  11. Klieneberger-Nobel E. 1950; Methods for the study of the cytology of bacteria and pleuropneumonia-like organisms. Quart. J. micr. Sci 91:340
    [Google Scholar]
  12. Kulka D., Walker T.K. 1948; Capsules of Aerobacter turbidans ‘demonstrated’ by positive staining. J. Inst.Brewing 54:148
    [Google Scholar]
  13. Leifson E. 1951; Staining, shape and arrangement of bacterial flagella. J. Bact 62:377
    [Google Scholar]
  14. Leifson E., Carhart S.R., Fulton M. 1955; Morphological characteristics of flagella of Proteus and related bacteria. J. Bact 69:73
    [Google Scholar]
  15. Leifson E., Hugh R. 1953; Variation in shape and arrangement of bacterial flagella. J. Bact 65:263
    [Google Scholar]
  16. Leifson E., Hugh R. 1954; A new type of polar monotrichous flagellation. J. gen. Microbiol 10:68
    [Google Scholar]
  17. Leifson E., Palen M.I. 1955; Variations and spontaneous mutations in the genus Listeria in respect to flagellation and motility. J. Bact 70:233
    [Google Scholar]
  18. Mackie T.J., Mccartney J.E. 1949; Handbook of Practical Bacteriology, 8th ed. Edinburgh: E. and S. Livingstone Ltd
    [Google Scholar]
  19. Mckinney R.E. 1953; Staining bacterial polysaccharides. J. Bact 66:453
    [Google Scholar]
  20. Methods 1954, 11th ed.. Prepared by Conn H. J., Bartholomew J. W., Jennison M. W. Geneva, New York: Biotech.Publications.;
  21. Niel C.B.van, Allen M.B. 1952; A note on Pseudomonas stutzeri. . J. Bact 64:413
    [Google Scholar]
  22. Novelli A. 1953; New method of staining of bacterial capsules in films and sections. Experientia 9:34
    [Google Scholar]
  23. Pijper A. 1946; Shape and motility of bacteria. J. Path. Bact 58:325
    [Google Scholar]
  24. Pijper A., Abraham G. 1954; Wavelengths of bacterial flagella. J. gen. Microbiol 10:452
    [Google Scholar]
  25. Pijper A., Neser M.L., Abraham G. 1956; The wavelengths of helical bacterial flagella. J. gen. Microbiol 14:371
    [Google Scholar]
  26. Rhodes M.E. 1957; The preservation of Pseudomonas under mineral oil. J. Appl. Bact 20:108
    [Google Scholar]
  27. Shewan J.M., Hodgkiss W., Liston J. 1954; A method for the rapid differentiation of certain non-pathogenic asporogenous bacilli. Nature; Lond.: 173208
    [Google Scholar]
  28. Sneath P.H.A. 1956a; Cultural and biochemical characteristics of the genus Chromobaderium. . J. gen. Microbiol 15:70
    [Google Scholar]
  29. Sneath P.H.A. 1956b; The change from polar to peritrichous flagellation in Chromobaderium spp. J. gen. Microbiol 15:99
    [Google Scholar]
  30. Weibull C. 1951; Movement of bacterial flagella. Nature; Lond.: 167511
    [Google Scholar]
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