RT Journal Article SR Electronic(1) A1 Jones, A. S. A1 Rizvi, S. B. H. A1 Stacey, M.YR 1958 T1 The Phosphorus-containinǵ Compounds of Gram-positive and Gram-negative Orǵanisms in Relation to the Gram Staininǵ Reaction JF Microbiology, VO 18 IS 3 SP 597 OP 606 DO https://doi.org/10.1099/00221287-18-3-597 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The phosphorus-containing compounds of certain Gram-positive and Gram-negative organisms were estimated by a modification of the Schmidt & Thannhauser (1945) procedure. Another method Mitchell & Moyle, 1950, 1951a, b, 1954 in which pentosenucleic acid (PNA) was estimated from the optical absorption of the ‘PNA fraction’ at 260 mμ. was found to be inaccurate, because of the presence of ultraviolet-absorbing impurities. A more accurate method for estimating PNA has now been developed, in which the nucleotides are separated from impurities by paper electrophoresis. The results thus obtained show that non-nucleotide phosphorus-containing compounds are present in the ‘PNA fraction’ of both Grampositive and Gram-negative organisms. There is, therefore, no evidence to connect these compounds with the Gram staining properties of Gram-positive microorganisms (cf. Mitchell & Moyle, 1950, 1951a, b, 1954)., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-18-3-597