1887

Abstract

SUMMARY: After irradiation of strain B by ultraviolet or ionizing radiations, different plating media give rise to widely different viable counts; the surviving fractions are smallest on media which are optimal for the growth of unirradiated organisms. It is concluded that inhibition of colony formation after irradiation is due in part to an injury which leads to imbalance in the synthetic processes of the cell, so that ‘recovery’ or ‘restoration’ may be brought about by suboptimal conditions of growth. The proportion of cell deaths which may be ascribed to this particular injury is determined by the conditions obtaining in bacterial suspensions before and during irradiation, and also by the type of radiation used.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-18-2-461
1958-04-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/18/2/mic-18-2-461.html?itemId=/content/journal/micro/10.1099/00221287-18-2-461&mimeType=html&fmt=ahah

References

  1. Alper T. 1955; Bacteriophage as indicator in radiation chemistry. Radiation Res 2:119
    [Google Scholar]
  2. Alper T., Gillies N.E. 1957; The dependence of the observed oxygen effect on the post-irradiation treatment of micro-organisms. Nature; Lond.: in the Press
    [Google Scholar]
  3. Anderson E.H. 1951; Heat reactivation of ultraviolet inactivated bacteria. J. Bact 61:389
    [Google Scholar]
  4. Barner H.D., Cohen S.S. 1956; The relation of growth to the lethal damage induced by ultraviolet irradiation in Escherichia coli. . J. Bact 71:149
    [Google Scholar]
  5. Buzzell A. 1956; The interrelationships between ultraviolet irradiation and the thermal reactivation of Escherichia coli B. Arch. Biochem. Biophys 62:97
    [Google Scholar]
  6. Ebert M. 1955; Hydrogen peroxide production under varying conditions of irradiation. In Radiobiology Symposium 1954, Proceedings of the Symposium held at Liège August-September 1954 pp 30–8 Ed. Bacq Z. M., Alexander P. London: Butterworths;
    [Google Scholar]
  7. Ellison S.A., Erlanger B.F., Allen P. 1955; The chemical reversal of ultraviolet effects on bacteria. J. Bact 69:536
    [Google Scholar]
  8. Heinmets F., Kathan R.H. 1954; Preliminary studies on the mechanism of biological action of ultraviolet irradiation and metabolic recovery phenomenon. Arch. Biochem. Biophys 53:205
    [Google Scholar]
  9. Heinmets F., Lehman J.J., Taylor W.W., Kathan R.H. 1954; The study of factors which influence metabolic reactivation of the ultraviolet inactivated Escherichia coli. . J. Bact 67:511
    [Google Scholar]
  10. Howard-Flanders P. 1956; Factors in the production of intense hydrogen ion beams. Ph.D. Thesis Univ ersity of London;
    [Google Scholar]
  11. Howard-Flanders P., Alper T. 1957; The sensitivity of micro-organisms to irradiation under controlled gas conditions. Radiation Res in the Press
    [Google Scholar]
  12. Latakjet R. 1943; Action du froid sur la réparation des radiolésions chez une levure et chez un bactérie. C.R. Acad. Sci., Paris 217:186
    [Google Scholar]
  13. Latarjet R. 1954; Spontaneous and induced cell restorations after treatments with ionizing and non-ionizing radiations. Acta radiol., Stockh 41:84
    [Google Scholar]
  14. Lederberg J., Lederberg E.M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact 63:399
    [Google Scholar]
  15. Miller N., Wilkinson J. 1952; Actinometry of ionizing radiations. In Radiation Chemistry, Disc. Faraday Soc12 p. 50
    [Google Scholar]
  16. Roberts R.B., Aldous E. 1949; Recovery from ultraviolet irradiation in Escherichia coli. . J. Bact 57:363
    [Google Scholar]
  17. Stapleton G.E., Billen D., Hollaender A. 1953; Recovery of X-irradiated bacteria at sub-optimal incubation temperatures. J. cell. comp. Physiol 41:345
    [Google Scholar]
  18. Stapleton G.E., Sbarra A.J., Hollaender A. 1955; Some nutritional aspects of bacterial recovery from ionizing radiations. J. Bact 70:7
    [Google Scholar]
  19. Stein W., Meutzner I. 1950; Reaktivierung von UV-inaktiviertem Bakterium coli durch Warme. Naturwissenschaften 37:167
    [Google Scholar]
  20. Wainwright S.D., Mullaney J. 1953; Some effects of metabolic inhibitors upon survival of ultraviolet irradiated Escherichia coli. . Experientia 9:376
    [Google Scholar]
  21. Wainwright S.D., Nevill A. 1955; Modification of the biological effects of ultraviolet irradiation by post-irradiation treatment with iodoacetate and peptone. J. gen. Microbiol 12:1
    [Google Scholar]
  22. Weatherwax R.S. 1956; Reactivation of ultraviolet-irradiated Escherichia coli. . J. Bact 72:329
    [Google Scholar]
  23. Witkin E.M. 1956; Time, temperature and protein synthesis: A study of ultraviolet-induced mutation in bacteria. Cold Spr. Harb. Symp. quant. Biol 21:123
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-18-2-461
Loading
/content/journal/micro/10.1099/00221287-18-2-461
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error