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SUMMARY: The effect of the ionic composition of the medium on the respiration of resting-cell suspensions of two Gram-negative micro-organisms has been investigated. Potassium or rubidium salts with Cl−, Br− or I− as anion were found to be necessary for the maximal rates of oxidation of glutamate or glucose by Pasteurella tularensis, and KC1 or K2S04 by Escherichia coli. The pH value of the medium and the concentration of the substrate influenced the effect of the electrolytes. Under certain conditions, a synergistic effect of Na+ ion was observed. The same ion mixtures which supported the respiration caused the maximal swelling of the bacterial cells.
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