1887

Abstract

SUMMARY: requires light intensities < 300 foot-candles and grows at 10°–33°. The medium developed for is representative of the early types of artificial media having a relatively high concentration of chelator and, necessarily, of trace metals. Other media, varying widely in trace metal: chelator ratios and concentrations allow growth of Tris 2-amino-2- hydroxymethylpropane-1:3-diol is a suitable pH buffer; its antagonism of potassium utilization was demonstrated and was amply overcome by the addition of 200 mg. K/l. to the medium. Inorganic and organic phosphates were utilized and were required at relatively low concentrations. P. utilized NO and NH but not atmospheric nitrogen. NaNO was the most satisfactory inorganic nitrogen source but the cultures bleached after prolonged growth. Asparagine (0·2–0·8 g./l.) maintained the normal purple pigmentation over a longer period; it served as a non-toxic slowly-utilized reservoir of available N. Vitamin B is the only essential vitamin and is needed at a concentration (100 mg./l.) which is higher than for most algae. All the cobalamins including factor B, but not methionine or deoxy- ribosides, can substitute for vitamin B. The physiological and ecological resemblances of P. to red or violet blue-green algae growing in deep water are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-18-1-190
1958-02-01
2024-11-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/18/1/mic-18-1-190.html?itemId=/content/journal/micro/10.1099/00221287-18-1-190&mimeType=html&fmt=ahah

References

  1. Boresch K. 1921; Die Komplementäre chromatische Adaptation. Arch. Protistenk 44:1
    [Google Scholar]
  2. Cowperthwaite J., Weber M. M., Packer L., Hutner S. H. 1953; Nutrition of Herpetomonas (Strigomonas) culcidarum . Ann. N. Y. Acad. Sci 56:972
    [Google Scholar]
  3. Dangeard P. A. 1911; Sur les conditions de l’assimilation chlorophylliene chez les Cyanophycées. C.R. Acad. Sci., Paris 152:967
    [Google Scholar]
  4. Droop M. R. 1955; A pelagic marine diatom requiring cobalamin. J. mar. biol. Ass. U.K 34:229
    [Google Scholar]
  5. Feldmann J. 1932; Sur la biologie des Trichodesmium Ehrenberg. Revue Algol 6:357
    [Google Scholar]
  6. Fogg G. E. 1942; Studies on nitrogen fixation by blue-green algae. I. Nitrogen fixation by Anabeana cylindria Lemm. J. exp. Biol 19:78
    [Google Scholar]
  7. Fogg G. E., Wolfe M. 1954; The nitrogen metabolism of the blue-green algae. Symp. Soc. gen. Microbiol 4:99
    [Google Scholar]
  8. Föyn B. 1934; Lebenszyklus, Cytologie und Sexualitat der Chlorophycae Cladophora suhriana, Kutzing. Arch. Protistenk 83:1
    [Google Scholar]
  9. Fritsch F. E. 1945 The Structure and Reproduction of the Algae II Cambridge: University Press;
    [Google Scholar]
  10. Harder R. 1922; Liehtintensität und ehromatische Adaptation bei den Cyanophyceen. Ber. dtsch. bot. Ges 40:26
    [Google Scholar]
  11. Haxo F., O’Heocha C., Norris P. 1955; Comparative studies of chromatographically separated phycoerythrins and phycocyanins. Arch. Biochem. Biophys 54:162
    [Google Scholar]
  12. Haxo F., Strout P. 1950; Nitrogen deficiency and coloration in red algae. Biol. Bull., Wood’s Hole 99:360
    [Google Scholar]
  13. Hutner S. H., Provasoli L., Filfus J. 1953; Nutrition of some phagotrophic fresh-water Chrysomonads. Ann. N.Y. Acad. Sci 56:852
    [Google Scholar]
  14. Kinne-Diettrich E-M. 1955; Beiträge zur Kenntnis der Ernährungsphysiologie mariner Blaualgen. Kieler Meeresforsch 11:34
    [Google Scholar]
  15. Kylin H. 1937; Über die Farbstoffe und die Farbe der Cyanophyceen. Forhand. K. Fysiogr. Sallsk. Lund 7:12
    [Google Scholar]
  16. MacLeod R. A., Onofrey E. 1954; Cation antagonism of the antibacterial action of amines. J. biol. Chem 210:193
    [Google Scholar]
  17. Provasoli L. 1957; Growth factors in unicellular marine algae: Scripps Symposium on ‘Perspectives in Marine Biology’ . University of California Press; In the Press
    [Google Scholar]
  18. Provasoli L., McLaughlin J. J. A., Droop M. R. 1957; The development of artificial media for marine algae. Arch. Mikrobiol 25:392
    [Google Scholar]
  19. Provasoli L., McLaughlin J. J. A., Pintner I. J. 1954; Relative and limiting concentrations of major mineral constituents for the growth of algal flagellates. Trans. N.Y. Acad. Sci 16:412
    [Google Scholar]
  20. Provasoli L., Pintner I. J. 1954; Cultural characteristics of Phormidium persicinum, an auxotrophic marine red-pigmented blue-green alga. Rapports et Comm, du VIII Congres Inter, de Bot. Sect 17:39
    [Google Scholar]
  21. Ridgway R. 1912 Color standards and Color Nomenclature Washington, D.C: Ridgway;
    [Google Scholar]
  22. Shilo M., Shilo M. 1953; Conditions which determine the efficiency of ammonium sulphate in the control of Prymnesiumparvum in fish breeding ponds. Appl. Microbiol 1:330
    [Google Scholar]
  23. Wille N. 1922; Phycoerythrinbei den Myxophyceen. Ber. dtsch. bot. Ges 40:188
    [Google Scholar]
/content/journal/micro/10.1099/00221287-18-1-190
Loading
/content/journal/micro/10.1099/00221287-18-1-190
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error