1887

Abstract

SUMMARY: The genetic investigation of forty-three phenotypically classified cysteineless mutants of by means of transduction suggests that in forty-one of the mutants, strains of similar phenotype result from mutations occurring at a series of sites which form a closely linked group, presumably situated within a single gene locus. Five such loci have been identified.

Only two of these five presumptive loci are linked, and within these linked loci an unusually high proportion of mutations to auxotrophy appear to result from a chromosomal deficiency. Another locus which is linked to an array of loci controlling tryptophan biosynthesis shows biochemical heterogeneity within the locus.

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/content/journal/micro/10.1099/00221287-18-1-154
1958-02-01
2021-08-01
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References

  1. Adams M. A. 1950; Methods for the study of bacterial viruses. Meth. med. Res 2:1
    [Google Scholar]
  2. Benzer S. 1955; Fine structure of a genetic region in bacteriophage. Proc. nat. Acad. Sci., Wash 41:344
    [Google Scholar]
  3. Benzer S. 1957; The elementary units of heredity. Symposium:. ‘The Chemical Basis of Heredity Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  4. Clowes R. C. 1958; Nutritional studies of cysteineless mutants of Salmonella typhimurium . J. gen. Migrobiol 18:140
    [Google Scholar]
  5. Demerec M. 1955; ‘What is a gene?’ Twenty years later. Amer. Nat 89:5
    [Google Scholar]
  6. Demerec M. 1956a; Terminology and Nomenclature. In Genetic Experiments with Bacteria. Pubh Carneg. Inst 612:1
    [Google Scholar]
  7. Demerec M. 1956b; A comparable study of certain gene loci in Salmonella. Cold Spr. Harb. Symp. quant. Biol 21:113
    [Google Scholar]
  8. Demerec M., Blomstrand I., Demerec Z. E. 1955; Evidence of complex loci in Salmonella. Proc. nat. Acad. Sci.,Wash 41:359
    [Google Scholar]
  9. Demerec M., Demerec Z. E. 1956; Analysis of linkage relationships in Salmonella by transduction techniques. In Brookhaven Symp. Biol 8:75
    [Google Scholar]
  10. Demerec M., Hartman Z. 1956; Tryptophan mutants in Salmonella typhimurium . In Genetic Studies with Bacteria. Publ. Carneg. Inst 612:5
    [Google Scholar]
  11. Demerec M., Moser H., Hemmerly J., Blomstrand I., Demerec Z. E., Fitzgerald P. L., Glover S. W., Hanson J. F., Nielson F. J., Yura T. 1954; Bacterial Genetics. I. Yr. Bk. Carneg. Inst 53:225
    [Google Scholar]
  12. Hartman P. E. 1956; Linked loci in the control of consecutive steps in the primary pathway of histidine synthesis in Salmonella typhimurium . In Publ. Carneg. Inst 612:35
    [Google Scholar]
  13. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in the growth of bacteriophage. J. gen. Physiol 36:39
    [Google Scholar]
  14. Hockenhull D. J. D. 1949; The sulfur metabolism of mold fungi. The use of biochemical mutant strains of Aspergillus nidulans in elucidating the bio-synthesis of cystine. Biochem. biophys. Acta 3:326
    [Google Scholar]
  15. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact 63:399
    [Google Scholar]
  16. Ozeki H. 1956; Abortive transduction in purine-requiring mutants of Salmonella typhimurium . In Genetic Studies with Bacteria. Publ. Carneg. Inst 612:97
    [Google Scholar]
  17. Pontecorvo G. 1952; Genetical analysis of cell organisation. Symp. Soc. exp. Biol 6:218
    [Google Scholar]
  18. Stocker B. A. D., Zinder N. D., Lederberg J. 1953; Transduction of flagellar characters in Salmonella. J. gen. Microbiol 9:410
    [Google Scholar]
  19. Yanofsky C. 1957; Enzymatic studies with a series of tryptophan mutants of Escherichia coli . J. biol. Chem 224:783
    [Google Scholar]
  20. Yura T. 1956; Evidence of non-identical alleles in Purine-requiring mutants of Salmonella. In Genetic Studies with Bacteria. Publ. Carneg. Inst 612:77
    [Google Scholar]
  21. Zinder N. D. 1955; Bacterial transduction. Symp. Genetic Recombination. J.cell. comp. Physiol 45: Suppl 2–23
    [Google Scholar]
  22. Zinder N. D., Lederberg J. 1952; Genetic exchange in Salmonella. J. Bact 64:679
    [Google Scholar]
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