@article{mbs:/content/journal/micro/10.1099/00221287-18-1-129, author = "Crumpton, M. J. and Davies, D. A. L. and Hutchison, Ann M.", title = "The Serological Specificities of Pasteurella pseudotuberculosis Somatic Antigens", journal= "Microbiology", year = "1958", volume = "18", number = "1", pages = "129-139", doi = "https://doi.org/10.1099/00221287-18-1-129", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-18-1-129", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: The serological specificities of lipopolysaccharides obtained from representatives of each of the five serological groups of Pasteurella pseudotuberculosis have been examined by haemagglutination methods. At concentrations of 500 μg./ml. solutions of the lipopolysaccharides failed to give full sensitization of erythrocytes, but after treatment with alkali full sensitization was obtained at concentrations in the range of 50 to 3 μg./ml. By using antisera prepared in rats by immunization with living P. pseudotuberculosis, the capacity of each ‘Smooth’ lipopolysaccharide to inhibit the agglutination of sensitized erythrocytes by each group antiserum was determined. Absolute group specificity was found by this method. It is considered that the lipopolysaccharides bear the specificity of the somatic antigens on which the serological grouping of the species is based. ‘Smooth’ P. pseudotuberculosis lipopoly-saccharides failed to inhibit the agglutination by P. pestis antisera of erythrocytes sensitized with P. pestis lipopolysaccharide, but inhibition of this system was obtained with lipopolysaccharides of ‘Rough’ P. pseudotuberculosis strains derived from various serological groups. These ‘Rough’ lipopolysaccharides were closely related serologically to one another and to the P. pestis product but were not identical in specificity. It is considered that in Smooth P. pseudotuberculosis strains the ‘Smooth’ somatic antigen does not overlie a ‘Rough’ somatic antigen.", }