@article{mbs:/content/journal/micro/10.1099/00221287-17-3-678, author = "Di Menna, Margaret E.", title = "The Isolation of Yeasts from Soil", journal= "Microbiology", year = "1957", volume = "17", number = "3", pages = "678-688", doi = "https://doi.org/10.1099/00221287-17-3-678", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-17-3-678", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: A series of comparative studies was made of soil yeasts in a yellow-brown earth originally under forest but now under pasture. Of six solid acid media used for primary isolations, an agar medium containing 4 % (w/v) glucose and 1 % (w/v) peptone gave the highest yeast counts. Two soil-extract agars and a modified Czapek-Dox agar gave slightly lower counts; a third soil-extract agar inhibited all but one yeast species and gave a much decreased count. The addition of a surface active agent to glucose peptone agar did not increase the count. Acid broth enrichment cultures gave a distorted picture of the species pattern shown by primary cultures on solid media. Prolonged mechanical shaking of soil dilutions before culturing, and the addition of a surface active agent to the diluent did not affect the yeast pattern seen, quantitatively or qualitatively.", }