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SUMMARY: The ‘bulging factor’, a new antibiotic with a chemical nature related to streptothricin, is produced by a Streptomyces strain and was isolated as reineckate and picrate. A study on its mode of action resulted in the development of a plate technique for assaying bulging factor activity. Evidence is presented in support of a theory about the existence of an active water-excreting mechanism in fungi susceptible to the bulging factor.
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