1887

Abstract

SUMMARY: A purified cellular polysaccharide was obtained from a non-capsulated pneumococcus ( strain R19, derived from a Type II organism). The main purification steps were peptic, tryptic and ribonuclease digestions, removal of protein by the Sevag technique and ethanol precipitation of the polysaccharide. Ribonuclease digestion was an essential step in the purification procedure. The constituent sugars of the isolated polysaccharide were galactose, mannose and glucosamine. The acetyl content of the polysaccharide indicated that the glucosamine was fully acetylated on the N position and that some O-acetyl was also present. The phosphorus content was not entirely explicable in terms of the known ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) contamination. Residual amino acid was consistently present which may have been due to bound amino acids or to protein contamination.

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/content/journal/micro/10.1099/00221287-17-2-437
1957-10-01
2022-01-20
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