1887

Abstract

SUMMARY: An osmotic barrier for phosphate very near the visible surface of the chick-embryo cell appears to regulate the reciprocal exchange of inorganic orthophosphate between the medium and a component of the acid-soluble inorganic phosphate of cells kept in monolayer culture. Some non-reciprocal transfer of inorganic phosphate occurs, which may or may not be due to cell damage, and the apparent phosphate-impermeable volume decreases by half after 3 hr. contact with phosphate at 0°. At 2° the exchange is at least 97 % inhibited. The phosphate- impermeable volume after cell rupture is less than 10 % of the intact-cell value, when internal phosphate is released in a form shown by mild separation to be mostly inorganic orthophosphate. Cells lose internal phosphate more slowly in absence of external phosphate, and the addition of external phosphate increases the loss rate to a maximum at physiological concentrations and higher.

Such a barrier provides information on the nature of the cell surface, and shows that adsorption, penetration and release of vesicular stomatitis virus occurs without any detectable damage to the surface controlling the phosphate-exchange rate.

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/content/journal/micro/10.1099/00221287-17-2-353
1957-10-01
2021-10-21
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References

  1. Bandurski R. S., Axelrod B. 1951; The chromatographic identification of some biologically important phosphate esters. J. biol. Chem 193:405
    [Google Scholar]
  2. Cooper P. D. 1957a; Some characteristics of vesicular stomatitis virus growth curves in tissue culture. J. gen. Microbiol 17:327
    [Google Scholar]
  3. Cooper P. D. 1957b; Paths of phosphate transfer in normal chick embryo cells and in cells infected with vesicular stomatitis virus. J. gen. Microbiol 17:335
    [Google Scholar]
  4. Dulbecco R. 1955; Interactions of viruses and animal cells. A study of facts and interpretations. Physiol. Rev 35:301
    [Google Scholar]
  5. Dulbecco R., Vogt M. 1954; One-step growth curve of western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells. J. exp. Med 99:183
    [Google Scholar]
  6. Greenberg D. M. 1952; The acid-soluble phosphates in animal metabolism. Phosphorus metabolism II p. 3 A symposium McElroy W. D., Glass B. Ed Baltimore: Johns Hopkins Press;
    [Google Scholar]
  7. Kamen M. D., Spiegelman S. 1948; Studies on the phosphate metabolism of some unicellular organisms. Cold Spr. Harb. Symp. quant. Biol 13:151
    [Google Scholar]
  8. Mitchell P. 1953; Transport of phosphate across the surface of M. pyogenes: nature of the cell ‘inorganic phosphate’. J. gen. Microbiol 9:273
    [Google Scholar]
  9. Mitchell P., Moyle J. 1953; Paths of phosphate transfer in M. pyogenes: phosphate turnover in nucleic acids and other fractions. J. gen. Microbiol 9:257
    [Google Scholar]
  10. Mitchell P., Moyle J. 1956; Osmotic function and structure in bacteria. In Bacterial Anatomy. Symp. Soc. gen. Microbiol 6:150
    [Google Scholar]
  11. Puck T. T., Lee H. H. 1954; Mechanism of cell wall penetration by viruses. I. An increase in host cell permeability induced by bacteriophage infection. J. exp. Med 99:481
    [Google Scholar]
  12. Sacks J. 1948; Mechanism of phosphate transfer across cell membranes. Cold Spr. Harb. Symp. quant. Biol 13:180
    [Google Scholar]
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