SUMMARY: When cultivated on membranes for electron microscopy, in the presence of 800 units penicillin/ml. and 10% (v/v) horse serum, a strain of developed L-colonies in two stages. In the first cultures, the colonies consisted of naked protoplasts with a wide periphery of relatively disorganized protoplasm, and when further subcultured upon penicillin the entire colonies consisted of disorganized protoplasm. The typical bacillary form could be recovered by subculture of this material upon penicillin-free medium. In the process of regeneration the cultures passed initially through a stage in which they again consisted of protoplasts.


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