1887

Abstract

SUMMARY: The optimal conditions for accurate and reproducible analysis of cellulose hydrolysis by micro-organisms, particularly from the rumen, were determined. Rumen micro-organisms were found to require approximately 50 mg. substrate (cellulose powder) in a 40 hr. incubation at 37° with 1 ml. of rumen liquor, and a total volume of 8 ml., in static vessels. All types of cellulose Or simpler substituted derivatives, from soluble carboxymethylcellulose through insoluble cellulose powder to cotton fibres, are nearly quantitatively hydrolysed by mixed rumen micro-organisms. The effect of the inorganic composition of the medium, of antibacterial agents (thymol, fluoride, toluene) and of concentration procedures on the cellulolytic activity of rumen micro-organisms was examined. Rumen protozoa appear to play a minor role in cellulose degradation; their effective activity is due mainly to closely associated, possibly ingested, bacteria. The validity of the results obtained by various methods of ‘cellulase ’ assay is discussed.

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/content/journal/micro/10.1099/00221287-17-1-153
1957-08-01
2021-10-22
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References

  1. Aitken R. A., Eddy B. P., Ingram M., Weurman C. 1956; The action of culture filtrates of thef ungus Myrothecium verrucaria on β-glucosans. Biochem. J 64:63
    [Google Scholar]
  2. Basu S. N., Pal P. N. 1956; Unfavourable effect of shaking on fungal cellulases. Nature; Lond: 178312
    [Google Scholar]
  3. Bryant M. P., Burkey L. A. 1953; Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci 36:205
    [Google Scholar]
  4. Elsden S. R. 1945; The fermentation of carbohydrates in the rumen of the sheep. J. exp. Biol 22:51
    [Google Scholar]
  5. Higa H. H., O’Neill R. D., Jennison M. W. 1956; Partial purification of cellulase from a wood rotting Basidiomycete. J. Bact 71:382
    [Google Scholar]
  6. Huffman G. W., Rabers P. A., Spriesterbach D. R., Smith F. 1955; Nature of a hemicellulose extracted from cellulose with water. Nature; Lond: 175990
    [Google Scholar]
  7. Hungate R. E. 1942; , The culture of Eudiplodinium neglectum, with experiments on the digestion of cellulose. Biol. Bull., Woods Hole 83:303
    [Google Scholar]
  8. Hungate R. E. 1943; Further experiments on cellulose digestion by protozoa in the rumen of cattle. Biol. Bull., Woods Hole 84:157
    [Google Scholar]
  9. Hungate R. E. 1950a; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev 14:1
    [Google Scholar]
  10. Hungate R. E. 1950b; Mutualisms in protozoa. Annu. Rev. Microbiol 4:53
    [Google Scholar]
  11. Jermyn M. A. 1952; Fungal cellulases. 1. General properties of unpurified enzyme preparations from Aspergillus oryzae. Aust. J. sci. Res. B 5:409
    [Google Scholar]
  12. Kitts W. D., Underkofler L. A. 1954; Digestion by rumen micro-organisms. Hydrolytic products of cellulose and the cellulolytic enzymes. J. Agric. Food Chem 2:639
    [Google Scholar]
  13. Masson F. M., Oxford A. E. 1951; The action of the ciliates of the sheep’s rumen upon various water soluble carbohydrates, including polysaccharides. J. gen. Microbiol 5:664
    [Google Scholar]
  14. Meites S., Burrell R. C., Sutton T. S. 1951; Factors influencing the in vitro digestion of cellulose by rumen liquor in presence of an antiseptic. J. Anim. Sci 10:203
    [Google Scholar]
  15. Nishizawa K., Kobayashi T. 1953; Cellulose splitting enzymes. 11. Mode of actio n of Irpex cellulase on its substrates. J. Agric. chem. Soc. Japan 27:239
    [Google Scholar]
  16. Oxford A. E. 1951; The conversion of certain soluble sugars to a glucosan by holotrich ciliates in the rumen of sheep. J. gen. Microbiol 5:83
    [Google Scholar]
  17. Phillipson A. T. 1953; Digestion of cellulose by the ruminant. In Biological Transformations of Starch and Cellulose. Symp. biochem. Soc 11:63
    [Google Scholar]
  18. Phillipson A. T., Cuthbertson D. P. 1956; Modern concepts of rumen digestion and metabolism. 7th Int. Congr. Anim. Husb., Madrid, Subject 6: p. 1
    [Google Scholar]
  19. Reese E. T., Siu R. G. H., Levinson H. S. 1950; The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis. J. Bact 59:485
    [Google Scholar]
  20. Sijpesteijn A. K. 1951; On Ruminococcus flavefaciens, a cellulose decomposing bacteria from the rumen of sheep and cattle. J. gen. Microbiol 5:869
    [Google Scholar]
  21. Siu R. G. H. 1951 Microbial Decomposition of Cellulose New York: Reinhold Publ. Corporation;
    [Google Scholar]
  22. Sugden B. 1953; The cultivation and metabolism of oligotrich protozoa from the sheep’s rumen. J. gen. Microbiol 9:44
    [Google Scholar]
  23. Thomas R. 1956; Fungal cellulases. VII. Stachybotrys atra: production and properties of the cellulolytic enzyme. Aust. J. biol. Sci 9:159
    [Google Scholar]
  24. Walseth C. S. 1952; Occurrence of cellulases in enzyme preparations from microorganisms. Tech. Pap., Pulp. Pap. Ind. N.Y 35:228
    [Google Scholar]
  25. Whitaker D. R. 1953; Purification of Myothecium verrucaria cellulase. Arch. Biochem. Biophys 43:253
    [Google Scholar]
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