1887

Abstract

SUMMARY: The optimal conditions for accurate and reproducible analysis of cellulose hydrolysis by micro-organisms, particularly from the rumen, were determined. Rumen micro-organisms were found to require approximately 50 mg. substrate (cellulose powder) in a 40 hr. incubation at 37° with 1 ml. of rumen liquor, and a total volume of 8 ml., in static vessels. All types of cellulose or simpler substituted derivatives, from soluble carboxymethylcellulose through insoluble cellulose powder to cotton fibres, are nearly quantitatively hydrolysed by mixed rumen micro-organisms. The effect of the inorganic composition of the medium, of antibacterial agents (thymol, fluoride, toluene) and of concentration procedures on the cellulolytic activity of rumen micro-organisms was examined. Rumen protozoa appear to play a minor role in cellulose degradation; their effective activity is due mainly to closely associated, possibly ingested, bacteria. The validity of the results obtained by various methods of ‘cellulase’ assay is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-17-1-153
1957-08-01
2020-01-24
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-17-1-153
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error