SUMMARY: A method of cultivating fungi on a membrane filter is described. The growth on the membrane appears to be more rapid than on solid media. After 2–3 days of growth a piece of the disk was dried and placed in immersion oil on a slide, thus rendering the membrane transparent and suitable for microscopic examination in transmitted light. The new method makes the use of plates, microcultures (slide cultures), etc., unnecessary. The mechanical breakage of the mycelial structures often encountered when species of mycelium have to be transferred to slides for microscopic examination is avoided. As only a small piece of the membrane is needed for microscopic examination, the growth on the membrane is allowed to continue as long as is required. Each time an inspection of the culture is required, a small piece of the disk is removed.


Article metrics loading...

Loading full text...

Full text loading...



  1. Gordon M. A., Ajello L., Georg L. K., Zeidberg L. D. 1952; Microsporum gypseum and Histoplasmac apsulatum spores in soil and water. Science 116:208
    [Google Scholar]
  2. Jannasch H. W. 1954; Beitrag zur Methode der direkten mikroskopischen Untersuchung von Mikroorganismen auf Membranfiltern: Über die Aufhellung der Filter. Zbl. Bakt. (1. Orig) 161:225
    [Google Scholar]
  3. Orlando M. D., Bolduan O. E. A. 1953; The application of the membrane filter to a variety of pathogenic bacteria and Fungi Imperfecti. Bact. Proc 53rd. Gen. Meet p. 26
    [Google Scholar]
  4. Tsuneishi N., Goetz A. 1953; Growth development and incubation of bacterial cultures on molecular filter membranes. Bact. Proc 53rd Gen. Meet p. 26
    [Google Scholar]

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error