1887

Abstract

SUMMARY: A method of cultivating fungi on a membrane filter is described. The growth on the membrane appears to be more rapid than on solid media. After 2–3 days of growth a piece of the disk was dried and placed in immersion oil on a slide, thus rendering the membrane transparent and suitable for microscopic examination in transmitted light. The new method makes the use of plates, microcultures (slide cultures), etc., unnecessary. The mechanical breakage of the mycelial structures often encountered when species of mycelium have to be transferred to slides for microscopic examination is avoided. As only a small piece of the membrane is needed for microscopic examination, the growth on the membrane is allowed to continue as long as is required. Each time an inspection of the culture is required, a small piece of the disk is removed.

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/content/journal/micro/10.1099/00221287-17-1-117
1957-08-01
2022-01-19
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References

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