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Abstract
SUMMARY: The staphylococcal typing-phage 42 D is propagated on bacteria which are lysogenic for the phage 1363, and the latter phage is present as a contaminant in preparations of phage 42 D. This contamination can be avoided by propagating a pure clone of phage 42 D on non-lysogenic organisms. Such nonlysogenic propagating strains can be isolated after the lysogenic propagating organisms have been irradiated heavily with ultraviolet radiation.
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© Society for General Microbiology, 1957