SUMMARY: The accumulation of pyruvate in cultures of grown with limiting concentrations of nicotinic acid has been investigated. Nicotinic acid-deficient organisms remaining in contact with a lactate medium under conditions where pyruvate accumulation is occurring gradually become deficient in thiamine. The thiamine content of organisms at various stages of growth was estimated, and a relationship between thiamine content and length of lag phase found. Maximum rates of pyruvate oxidation were obtained in organisms which were not deficient in either nicotinic acid or thiamine. The possible metabolic significance of these findings is discussed.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error