1887

Abstract

SUMMARY: A comparison of an indirect method of counting virus particles (i.e. the ‘absolute assay ’ haemagglutination method) with direct electron-microscope counts showed that the indirect method underestimated the total virus particle count by a factor of about 10. Details of technique and possible sources of error in making counts are discussed.

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/content/journal/micro/10.1099/00221287-16-3-668
1957-06-01
2022-06-29
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References

  1. Appleby J.C. 1952; The isolation and properties of a modified strain of neurotropic influenza A virus.. Brit. J. exp. Path. 33:280
    [Google Scholar]
  2. Burnet F.M., Edney M. 1951; Recombinant viruses obtained from double infections with the influenza A viruses MEL and Neuro-WS.. Aust. J. exp. Biol, med. Sci. 29:353
    [Google Scholar]
  3. Dawson I.M., Elford W.J. 1949; The investigation of influenza and related viruses in the electron microscope by a new technique.. J. gen. Microbiol. 3:298
    [Google Scholar]
  4. Donald H.B., Isaacs A. 1954; Counts of influenza virus particles.. J. gen. Microbiol. 10:457
    [Google Scholar]
  5. Francis T. Jun Moore A.E. 1940; A study of the neurotropic tendency in strains of the virus of epidemic influenza.. J. exp. Med. 72:717
    [Google Scholar]
  6. Horsfall F.L. Jun 1954; On the reproduction of influenza virus. Quantitative studies with procedures which enumerate infective and haemagglutinating virus particles.. J. exp. Med. 100:135
    [Google Scholar]
  7. Isaacs A., Donald H.B. 1955; Particle counts of haemagglutinating viruses.. J. gen. Microbiol. 12:241
    [Google Scholar]
  8. Levine S., Puck T.T., Sagik B.P. 1953; An absolute method for assay of virus haemagglutinins.. J. exp. Med. 98:521
    [Google Scholar]
  9. Luria S.E. 1953 General Virology pp. 53–54 New York:: John Wiley.;
    [Google Scholar]
  10. Luria S.E., Williams R.C., Backus R.C. 1951; Electron micrographic counts of bacteriophage particles.. J. Bact. 61:179
    [Google Scholar]
  11. Reed L.J., Muench H. 1938; A simple method of estimating fifty per cent end points.. Amer. J. Hyg. 27:493
    [Google Scholar]
  12. Tyrrell D.A.J. 1955; New tissue culture systems for influenza, Newcastle disease and vaccinia viruses.. J. Immunol. 74:293
    [Google Scholar]
  13. Werner G.H., Schlesinger R.W. 1954; Morphological and quantitative comparison between infectious and non-infectious forms of influenza virus.. J. exp. Med. 100:203
    [Google Scholar]
  14. Williams R.C., Backus R.C. 1949; Macromolecular weights determined by direct particle counting. I. The weight of the bushy-stunt virus particle.. J. Amer. chem. Soc. 71:4052
    [Google Scholar]
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