SUMMARY: In three independently obtained mutant strains of , resistant in different degrees to acriflavine, resistance is due, in each case, to mutation in a single gene. Two of the mutant alleles, 1 and 3, are semi-dominant and either allele confers a high degree of resistance. These alleles are located about 23 units distal to the (white conidia) locus and are presumably allelic. A cross involving these two alleles in repulsion gave 0.1% sensitives. A third mutant allele (2) is also located on the chromosome, but on the other arm about 25 units distal to the 1 locus and over 100 units distant from the 1 and 3 loci. This allele, which confers relatively slight resistance, is almost completely recessive. Diploid strains which carry any allele for resistance in heterozygous condition give, by vegetative segregation, haploid and homozygous diploid resistant types which are preferentially selected on medium with acriflavine. The use of this technique for the automatic selection of vegetative segregants provides an additional tool for analyses through the parasexual cycle.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error