1887

Abstract

SUMMARY: Radioactive glucose has been supplied to starved and unstarved cells of the fungus for short periods of time, and a partial analysis was made of the nature of those substances incorporating radiocarbon from labelled glucose. It was found that although glucose was metabolized more rapidly by unstarved cells it rapidly entered the cells in both nutritional states and was converted to a number of other substances within the first 15 sec. Differences were found in the pattern of incorporation of labelled carbon between starved and unstarved cells, suggesting that starvation resulted in a disturbance of the balance of the relative metabolic pathways utilized for glucose metabolism in unstarved cells.

In further experiments cells were prepared in four physiological states: growing, resting after growth, starved in buffer, and after metabolizing glucose for several hours subsequent to starvation. By use of substrates variously labelled with radiocarbon it was found that the extent of recycling in the tricarboxylic acid cycle, which is similar in growing and resting cells, rose considerably when the cells were starved, and remained high for several hours during the incubation of starved cells with glucose. The minimum percentage of glucose which was metabolized primarily at the Ci position rose from 11 % in growing cells to 19 % in resting cells, fell nearly to zero immediately following starvation, and increased to 5 % after some hours in the presence of glucose.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-16-3-550
1957-06-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/16/3/mic-16-3-550.html?itemId=/content/journal/micro/10.1099/00221287-16-3-550&mimeType=html&fmt=ahah

References

  1. Aronoff S., Vernon L. 1950; Metabolism of soybean leaves. I. The sequence of formation of soluble carbohydrates during photosynthesis. Arch. Biochem 28:424
    [Google Scholar]
  2. Bandurski R. S., Axelrod B. 1951; The chromatographic identification of some biologically important phosphate esters. J. biol. Chem 193:405
    [Google Scholar]
  3. Claridge C. A., Werkman C. H. 1954; Intermediates of the aerobic dissimilation of 2-ketogluconate by Pseudomonas aeruginosa . Arch. Biochem. Biophys 51:395
    [Google Scholar]
  4. DeMoss R. D. 1953; Routes of ethanol formation in bacteria. J. cell. comp. Physiol 41: Suppl. 1 207
    [Google Scholar]
  5. Hanes C. S., Isherwood F. A. 1949; Separation of the phosphoric esters on the filter paper chromatogram. Nature; Lond: 1641107
    [Google Scholar]
  6. Heath E. C., Koffler H. 1956; Biochemistry of filamentous fungi. II. The quantitative significance of an ‘oxidative pathway’. J. Bact 71:174
    [Google Scholar]
  7. Millbank J. W. 1954 A study of intermediary metabolism in two micro-organisms, with particular reference to the keto-acids Ph.D. Thesis University of London:
    [Google Scholar]
  8. Moses V. 1954; The effect of ammonia on the oxidation of glucose by Zygorrhynchus moelleri . Biochem. J 57:547
    [Google Scholar]
  9. Moses V. 1955a; Glucose respiration in Zygorrhynchus moelleri; the entry of glucose into the cells. J. exp. Bot 6:222
    [Google Scholar]
  10. Moses V. 1955b; The disappearance of intermediates involved in glucose oxidation during the starvation of the fungus Zygorrhynchus moelleri . Ann. Bot., Lond., N.S 19:211
    [Google Scholar]
  11. Moses V. 1955c; Tricarboxylic acid cycle reactions in the fungus Zygorrhynchus moelleri . J. gen. Microbiol 13:235
    [Google Scholar]
  12. Moses V. 1957; The metabolic significance of the citric acid cycle in the growth of the fungus Zygorrhynchus moelleri . J. gen. Microbiol 16:534
    [Google Scholar]
  13. Norris F. C., Campbell J. J. R. 1949; The intermediate metabolism of Pseudomonas aeruginosa. III. The application of paper chromatography to the identification of gluconic and 2-ketogluconic acids, intermediates in glucose oxidation. Canad. J. Res 27C:253
    [Google Scholar]
  14. Roberts R. B., Cowie D. B., Britten R., Bolton E., Abelson P. H. 1953; The role of the tricarboxylic acid cycle in amino acid synthesis in Escherichia coli . Proc. nat. Acad. Sci., Wash 39:1013
    [Google Scholar]
  15. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugar on paper chromatograms. Nature; Lond: 166444
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-16-3-550
Loading
/content/journal/micro/10.1099/00221287-16-3-550
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error