1887

Abstract

SUMMARY: A simple glass slide technique has been devised for the continuous microscopical observation of growth and infection of root hairs of clover seedlings. The method involves an aseptic cultivation of seedlings on microscope slides which are partly immersed in a mineral salts medium. The roots are protected by a cover-slip.

By this procedure, the root hairs of white clover inoculated with nodule bacteria were studied. The earliest infection was observed to take place within 48 hr. of inoculation, on 4-day-old seedlings. In branched hairs the growth of the thread from a lateral branch towards the hair tip is tentatively explained as an effect of the position of the hair nucleus relative to the site of infection.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-16-2-374
1957-04-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/16/2/mic-16-2-374.html?itemId=/content/journal/micro/10.1099/00221287-16-2-374&mimeType=html&fmt=ahah

References

  1. Beijerinck M. W. 1888; Die Bacterien der Papilionaceen-Knöllchen. Bot. Ztg 46:725–757
    [Google Scholar]
  2. Bieberdorf F. W. 1938; The cytology and histology of the root nodules of some Leguminosae. J. Amer. Soc. Agron 30:375
    [Google Scholar]
  3. Bryan W. H., Newcomb E. H. 1954; Stimulation of pectin methylesterase activity of cultured tobacco pith by indoleacetic acid. Physiol. Plant 7:290
    [Google Scholar]
  4. Chen H. K. 1938; Production of growth substance by clover nodule bacteria. Nature; Lond: 142753
    [Google Scholar]
  5. Chen H. K., Thornton H. G. 1940; The structure of ‘ineffective’ nodules and its influence on nitrogen fixation. Proc. Roy. Soc. B 129:208
    [Google Scholar]
  6. Dangeard P. A. 1926; Recherches sur les tubercules radicaux des Légumineuses. Botaniste 16:1
    [Google Scholar]
  7. Dawson M. 1900; Nitragin and the nodules of leguminous plants. Phil. Trans. B 192:1
    [Google Scholar]
  8. Ekdahl I. 1953; Studies on the growth and the osmotic conditions of root hairs. Synth, hot. upsaliens 11:6
    [Google Scholar]
  9. Eriksson J. 1874 Studier öfver Leguminosernas rotknölar Dissertation. Lund:
    [Google Scholar]
  10. Frank B. 1890 Über die Pilzsymbiose der Leguminosen Berlin: Parey;
    [Google Scholar]
  11. Georgi C. E., Beguin A. E. 1939; Heteroauxin production by efficient and inefficient strains of rhizobia. Nature; Land: 14325
    [Google Scholar]
  12. Lundegårdh H. 1946; The growth of root hairs. Ark. Bot 33A:5
    [Google Scholar]
  13. McCoy E. 1929; A cytological and histological study of the root nodules of the bean, Phaseolus vulgaris L. Zbl. Bakt. (II) 79:394
    [Google Scholar]
  14. McCoy E. 1932; Infection by Bact. radicicola in relation to the microchemistry of the host’s cell walls. Proc. Roy. Soc. B 110:514
    [Google Scholar]
  15. Neely W. B., Ball C. D., Hamner C. L., Sell H. M. 1950; Effect of 2,4-dichlorophenoxyacetic acid on the invertase, phosphorylase and pectin methoxylase activity in the stems and leaves of the red kidney bean plants. Plant Physiol 25:525
    [Google Scholar]
  16. Nutman P. S. 1946; Genetical factors concerned in the symbiosis of clover and nodule bacteria. Nature; Lond: 157463
    [Google Scholar]
  17. Nutman P. S. 1953; Studies on the physiology of nodule formation. IV. The mutual inhibitory effects on nodule production of plants grown in association. Ann. Bot., N.S 17:95
    [Google Scholar]
  18. Nutman P. S. 1956; The influence of the legume in root-nodule symbiosis. A comparative study of host determinants and functions. Biol. Rev 31:109
    [Google Scholar]
  19. Prazmowski A. 1890; Die Wurzelknöllchen der Erbse. Landw. VersSta 37:161
    [Google Scholar]
  20. Schaede R. 1940; Die Knöllchen der adventiven Wasserwurzeln von Neptunia oleracea und ihre Bakteriensymbiose. Planta 31:1
    [Google Scholar]
  21. Schaede R. 1941; Untersuchungen über die Wurzelknöllchen von Vicia faba und Pisum sativum . Beitr. Biol. Pfl 27:165
    [Google Scholar]
  22. Thimann K. V. 1936; On the physiology of the formation of nodules on legume roots. Proc. nat. Acad. Sci., Wash 22:511
    [Google Scholar]
  23. Thornton H. G. 1929; The role of the young lucerne plant in determining the infection of the root by the nodule-forming bacteria. Proc. Roy. Soc. B 104:481
    [Google Scholar]
  24. Thornton H. G. 1936; The action of sodium nitrate upon the infection of lucerne root-hairs by nodule bacteria. Proc. Roy. Soc. B 119:474
    [Google Scholar]
  25. Thornton H. G. 1954; The nodule bacteria and their host legumes: some problems that they still present. Sci. Progr 42:185
    [Google Scholar]
  26. Thornton H. G., Nicol H. 1936; Stimulation of root-hair growth in legumes by sterile secretions of nodule bacteria. Nature; Lond: 137494
    [Google Scholar]
  27. Viermann H. 1929; Die Wurzelknöllchen der Lupine. Bot. Arch 25:45
    [Google Scholar]
  28. Ward H. M. 1887; On the tubercular swellings on the roots of Vida faba . Phil. Trans. B 178:539
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-16-2-374
Loading
/content/journal/micro/10.1099/00221287-16-2-374
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error