1887

Abstract

Summary: A Gram-negative, non-flagellated, asporogenous, anaerobic, iodophilic, curved rod (2·5 μ. × 0·5 μ.) was isolated from the rumen of a sheep and designated n.sp. This organism transforms a number of carbohydrates including pentoses to intracellular iodophilic polysaccharide which has been isolated and shown to be a polymer of glucose. Resting suspensions of this organism utilize this iodophilic material as a reserve substance under a variety of conditions. grows optimally when bicarbonate and rumen liquor are included in the medium. On physiological and morphological grounds it is considered to be an authentic rumen bacterium. The chief fermentation product from xylose is butyric acid.

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1957-02-01
2024-04-18
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References

  1. Barker S.B., Summerson W.H. 1941; The colorimetric determination of lactic acid in biological material. J. biol. Chem. 138:536
    [Google Scholar]
  2. Barry V.C., Dillon T. 1940; Occurrence of xylans in marine algae. Nature; Lond.: 146620
    [Google Scholar]
  3. Bergey’s Manual of Determinative Bacteriology 1948 Breed R. S., Murray E. G., Hitchens A. P. Ed. , 6th ed.. Baltimore, Md.: Williams and Wilkins;
  4. Bryant M.P., Burkey L.A. 1953; Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205
    [Google Scholar]
  5. Bryant M.P., Doetsch R.N. 1954a; A study of actively cellulolytic rodshaped bacteria of the bovine rumen. J. Dairy Sci. 37:1176
    [Google Scholar]
  6. Bryant M. P., Doetsch R. N. 1954b; Factors necessary for growth of Bacteroides succinogenes in the volatile acid fraction of rumen fluid. Science 120:944
    [Google Scholar]
  7. Doetsch R.N., Robinson R.Q., Shaw J.C. 1952; Techniques employed in cultural investigations of the bacteriology of bovine rumen contents. J. Anim. Sci. 11:536
    [Google Scholar]
  8. Doetsch R.N., Robinson R.Q., Brown R.E., Shaw J.C. 1953; Catabolic reactions of mixed suspensions of bovine rumen bacteria. J.Dairy Sci. 36:825
    [Google Scholar]
  9. Eadie J.M., Oxford A.E. 1955; Factors involved in the production of a novel kind of derangement of storage mechanism in living holotrich ciliate protozoa from sheep rumen. J.gen. Microbiol. 12:298
    [Google Scholar]
  10. Fisher P.J., Conn J.E. 1942; A flagella staining technic for soil bacteria. Stain Tech. 17:117
    [Google Scholar]
  11. Forsyth G., Hirst E.L. 1953; Protozoal polysaccharides. Structure of the polysaccharide produced by the holotrich ciliates present in sheep’s rumen. J. chem. Soc. p. 2132
    [Google Scholar]
  12. Gibbons R.J., Doetsch R.N., Shaw J.C. 1955; Further studies on polysaccharide production by bovine rumen bacteria. J. Dairy Sci. 38:1147
    [Google Scholar]
  13. Heald P.J. 1952; The fermentation of pentoses and uronic acids by bacteria from the rumen contents of sheep. Biochem. J. 50:503
    [Google Scholar]
  14. Hirsch A., Grinsted E. 1954; Methods for the growth and enumeration of anaerobic spore-formers from cheese, with observations on the effect of nisin. J. Dairy Res. 21:101
    [Google Scholar]
  15. Hobson P.N., Macpherson M.J. 1952; Amylases of Clostridium butyricum and a Streptococcus isolated from the rumen of the sheep. Biochem. J. 52:671
    [Google Scholar]
  16. Hobson P.N., Mann S.O. 1955; Some factors affecting the formation of iodophilic polysaccharide in Group D streptococci from the rumen. J. gen. Microbiol. 13:420
    [Google Scholar]
  17. Howard B.H. 1955; Ruminal fermentation of pentosan. Biochem. J. 60:i
    [Google Scholar]
  18. Hughes W.H. 1956; The structure and development of the induced long forms of bacteria. In Bacterial Anatomy, 6th Symp. Soc. gen. Microbiol. Cambridge University Press;
    [Google Scholar]
  19. Huhtanen C.N., Gall L.S. 1953; Rumen organisms. I. Curved rods and a related rod type. J. Bact. 65:548
    [Google Scholar]
  20. Hungate R.E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev. 14:1
    [Google Scholar]
  21. James A.T., Martin A.J.P. 1952; Gas liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid. Biochem. J. 50:679
    [Google Scholar]
  22. Leifson E. 1951; Staining, shape, and arrangement of bacterial flagella. J. Bact. 62:377
    [Google Scholar]
  23. Lewis D. 1954; The reduction of sulphate in the rumen of the sheep. Biochem. J. 56:391
    [Google Scholar]
  24. Mann S.O., Oxford A.E. 1955; Relationships between viable saccharolytic bacteria in rumen and abomasum of the young calf and kid. J. gen. Microbiol. 12:140
    [Google Scholar]
  25. Mcneill J.J., Doetsch R.N., Shaw J.C. 1954; Some nutritional requirements of bovine rumen bacteria. J. Dairy Sci. 37:81
    [Google Scholar]
  26. Moir R.J., Masson M.J. 1952; An illustrated scheme for the microscopic identification of the rumen microorganisms of sheep. J. Path. Bact. 64:343
    [Google Scholar]
  27. Oxford A.E. 1951; Conversion of certain soluble sugars to a glucosan by holotrich ciliates in the rumen of the sheep. J. gen. Microbiol. 5:83
    [Google Scholar]
  28. Perlin A.S. 1951; Isolation and composition of the soluble pentosans of wheat flours. Cereal Chem. 28:370
    [Google Scholar]
  29. Plimmer H.J., Paine S.G. 1921; A new method for the staining of bacterial flagella. J. Path. Bact. 24:286
    [Google Scholar]
  30. Robinow C.F. 1945; Nuclear apparatus and cell structure of rod-shaped bacteria. In Bacterial Cell Cambridge, Mass: Harvard University Press;
    [Google Scholar]
  31. Robinson R.Q., Doetsch R.N., Sirotnak F.M., Shaw J.C. 1955; Production of lactic acid and an iodine staining substance by bovine rumen bacteria. J. Dairy Sci. 38:13
    [Google Scholar]
  32. Somogyi M. 1945; A new reagent for the determination of sugars. J. biol. Chem. 160:61
    [Google Scholar]
  33. Wilson M.K., Briggs C.A.E. 1955; The normal flora of the bovine rumen. II. Quantitative bacteriological studies. J. appl. Bact. 18:294
    [Google Scholar]
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