1887

Abstract

SUMMARY: Levansucrase in the corynebacterium studied is apparently an adaptive enzyme. Its optimal formation is dependent on the presence of an inducer (sucrose) and an organic nitrogen source. High nitrogen concentrations increase growth rate and sucrose hydrolysis and significantly decrease levansucrase formation. The pH activity curve of the levansucrase studied is asymmetric and the optimal pH value for its activity is 7·0. These findings suggest that this levansucrase differs from other levansucrases so far described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-15-3-462
1956-12-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/15/3/mic-15-3-462.html?itemId=/content/journal/micro/10.1099/00221287-15-3-462&mimeType=html&fmt=ahah

References

  1. Avineri-Shafiro S., Hestrih .S. 1945; The mechanism of polysaccharide formation from sucrose, 2. Biochem. J 39:167
    [Google Scholar]
  2. Bacon J. S. D., Edelman J. 1951; The carbohydrates of the Jerusalem Artichoke and other Compositae. Biochem. J 48:114
    [Google Scholar]
  3. Beijerinck M. W. 1912; Die durch Bakterien aus Rohzucker erzeugten schleimigen Wanstoffe. Folia microbiol., Delft 1:877
    [Google Scholar]
  4. Doudoroff M., O’Neal R. 1945; The reversibility of Levulan synthesis by Bacillus subtilis. J. biol. Chem 159:585
    [Google Scholar]
  5. Duguid J. P., Wilkinson J. F. 1953; The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. J. gen. Microbiol 9:174
    [Google Scholar]
  6. Hehre E. J. 1955 In Methods in Enzymology Colowick S. P., Kaplan N. O. Ed New York: Academic Press;
    [Google Scholar]
  7. Henis Y., Aschner M. 1954; Levan formation in a species of Corynebacterium. Bull. Res. Coun. Israel 4:209
    [Google Scholar]
  8. Hestrin S., Avineri-Shafiro S. 1944; The mechanism of polysaccharide formation from sucrose. Biochem. J 38:2
    [Google Scholar]
  9. Hestrin S., Avineri-Shapiro S., Aschner M. 1943; The enzymic production of levan. Biochem. J 37:450
    [Google Scholar]
  10. Hestrin S., Goldblum J. 1953; Levanpolyase. Nature; Lond: 1721046
    [Google Scholar]
  11. Karstrom H. 1938; Enzymatische Adaptation beiMikroorganismen, in: Ergebnisse der Enzymforschung. 7350 Leipzig: Akademischer Verlag;
    [Google Scholar]
  12. Mehl J. W. 1945; The biuret reaction of proteins in the presence of ethylene glycol. J. biol. Chem 157:173
    [Google Scholar]
  13. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. J. biol. Chem 153:375
    [Google Scholar]
  14. Partridge S. M. 1948; Filter paper partition chromatography of sugars. Biochem. J 42:238
    [Google Scholar]
  15. Somogyi M. 1945; A new reagent for the determination of sugars. J. biol. Chem 160:61
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-15-3-462
Loading
/content/journal/micro/10.1099/00221287-15-3-462
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error