The Essential Metabolites of a Strain of Paramecium aurelia (Stock 47.8) and a Comparison of the Growth Rate of Different Strains of Paramecium aurelia in Axenic Culture
SUMMARY:Paramecium aurelia, var. 4, stock 47.8 (sensitive) grown in an axenic medium required the following amino acids: dl-tyrosine, l-phenylalanine, l-tryptophan, dl-methionine, dl-threonine, l-leucine, dl-isoleucine, l-lysine, l-histidine, l-arginine, and dl-serine. dl-valine, l-proline and glycine were synthesized at a slow rate. Tyrosine, methionine and serine were required by stock 51.7 (sensitive). Other essential metabolites required by stock 47.8 (s) were thiamine, riboflavin, folic acid, nicotinic acid, pantothenic acid, and possibly pyridoxal. 2,6-diaminopurine inhibited growth at concentrations as low as 1–4 μg./ml. The growth rates (expressed as final population density/ml./transfer) of various stocks in identical media are different. An apparent correlation was found to exist between the growth rates and the chromosome number of these stocks, those with the largest chromosome number attaining the highest population density.
ConnerR.L.,
Van WagtendonkW.J.,
MillerC.A.1953; The isolation from lemon juice of a growth factor of steroid nature required for the growth of a strain of Paramecium aurelia. J. gen. Microbiol. 9:434
Van WagtendonkW.J.,
ConnerR.L.,
MillerC.A.,
RaoM.R.R.1953; Growth requirements of Paramecium aurelia, var. 4, stock 51, sensitives and killers, in axenic medium. Ann. N.Y. Acad. Sci. 56:929
Van WagtendonkW.J.,
MillerC.A.,
ConnerR.L.1952; Growth requirements of Paramecium aurelia, var. 4, stock 51.7 (s), in a medium free of other living organisms. Fed. Proc. 11:302
WeissE.D.,
BallG.H.1947; Nutritional requirements of Tritrichomonas foetus with special reference to partially digested proteins. Proc. Soc. exp. Biol., N.Y. 65:278
The Essential Metabolites of a Strain of Paramecium aurelia (Stock 47.8) and a Comparison of the Growth Rate of Different Strains of Paramecium aurelia in Axenic Culture