1887

Abstract

Summary: Cysteine was formed from sulphate, sulphite and thiosulphate as inorganic sulphur sources, by acetone-dried powders of mycelium. Added pyridoxal was obligatory for cysteine synthesis with sulphate or sulphite, and this synthesis was further enhanced by the addition of sodium pyruvate and sodium glutamate. Concomitant accumulation of cysteine sulphinic acid and utilization of glutamic acid was demonstrated. With thiosulphate, considerable synthesis of cysteine occurred in the absence of supplements, but was much enhanced by the addition of serine. Cysteine sulphinic acid did not accumulate with thiosulphate as the inorganic sulphur source. Parathiotrophic mutants, produced by ultraviolet irradiation, were used in the further elucidation of the biosynthetic mechanisms involved. Two reaction sequences: sulphate →sulphite →cysteine sulphinic acid →cysteine; and thiosulphate →cysteine-S-sulphonic acid →cysteine probably take place in the mould.

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1956-08-01
2022-01-27
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References

  1. Chapeville F., Fraomageot P. 1954; La formation enzymatique de l'acide cysteinesulphinique a partir de sulfite. Biochim. biophys.Acta 14:415
    [Google Scholar]
  2. Clarke H.J. 1932; The action of sulphite upon cysteine. J. biol. Chem. 97:235
    [Google Scholar]
  3. Cowie D.B., Bolton E.T., Sands M.K. 1950; Sulphur metabolism in Escherichia coli. I. Sulphate metabolism of normal and mutant cells. J. Bact. 60:233
    [Google Scholar]
  4. Fildes P., Richardson G.M. 1937; The nutrition of Staphylococcus aureus: sulphur requirements. Brit.J. exp. Path. 18:292
    [Google Scholar]
  5. Fries N. 1945; X-ray induced mutations in the physiology of Opkiostoma. Nature; Lond.: 155757
    [Google Scholar]
  6. Fromageot C., Wookey E., Chaix P. 1940; Sur la degradation anaerobie de la cysteine par la desulphurase du foie. Enzymologia 28:108
    [Google Scholar]
  7. Hockenhull D.J.D. 1948; Studies in penicillin production by Penicillium notatum in surface culture: II. Further studies in the metabolism of sulphur. Biochem. J. 43:408
    [Google Scholar]
  8. Hockenhull D.J.D. 1949; The sulphur metabolism of mould fungi: the use of ‘biochemical mutant’ strains of Aspergillus nidulans in elucidating the biosynthesis of cystine. Biochim. biopkys.Acta 3:826
    [Google Scholar]
  9. Hughes D.E. 1951; A press for disrupting bacteria and other microorganisms. Brit.J. exp. Path. 32:07
    [Google Scholar]
  10. Kassell B., Brand E. 1938; The photometric determination of cystine, cysteine, ascorbic acid and related compounds with phosphotungstic acid. J. biol. Chem. 125:115
    [Google Scholar]
  11. Kearney E.B., Singer T.P. 1952; Intermediary metabolism of cysteine sulphinic acid in cell-free extracts of Proteus vulgaris. . Biochim. biopkys.Acta 8:608
    [Google Scholar]
  12. Kearney E.B., Singer T.P. 1953; The oxidation of cysteine sulphinic and cysteic acids in Proteus vulgaris. Biochim. biophys.Acta 11:270
    [Google Scholar]
  13. Kharasch M.S., Brown H.C. 1940; Chlorinations with sulfuryl chloride. III. (a) The peroxide catalysed chlorination of aliphatic acids and acid chlorides. (b) The photochemical sulphonation of aliphatic acids. J. Amer. chem. Soc. 62:025
    [Google Scholar]
  14. Lampen J.O., Roepke R.R., Jones M.J. 1947; Studies on the sulphur metabolism of Escherichia coli. III. Mutant strains of Escherichia coli unable to utilize sulphate for their complete sulphur requirements. Arch. Biochem. 13:55
    [Google Scholar]
  15. Levine T.F. 1936; The oxidation of cystine in non-aqueous media. VI. A study of the reactions of the disulphoxide of L-cystine, especially of its dismutative decompositions. J. biol. Chem. 113:571
    [Google Scholar]
  16. McIlwain H., Roper J.A., Hughes D.E. 1948; Relationships in streptococci between growth and metabolism of glutamine. Biochem.J. 42:402
    [Google Scholar]
  17. Medes G., Floyd N. 1942; Metabolism of sulphur. II. Further investigations of the enzymic oxidation of sulphur-containing amino acids. Biochem.J. 36:250
    [Google Scholar]
  18. Mickle H. 1948; Tissue disintegrator. J. R. micr. Soc. 68:10
    [Google Scholar]
  19. Phinney B.O. 1948 Abstract in Genetics 33:624
    [Google Scholar]
  20. Phinney B.O., Fling M., Sheng T.C., Horowitz N.H. 1950; Unpublished experiments quoted in ‘Biochemical genetics of Neurospora’ by Horowitz, N.H. Advanc. Genet. 3:33
    [Google Scholar]
  21. Pontecorvo G. 1949; Auxanographic techniques in biochemical genetics. J. gen. Microbiol. 3:122
    [Google Scholar]
  22. Pontecorvo G. 1953; The genetics of Aspergillus nidulans. Advanc. Genet. 5:141
    [Google Scholar]
  23. Robertson W.B. 1942; The preparation of sodium pyruvate. Science 96:93
    [Google Scholar]
  24. Shinohara K. 1932; Oxidation of cystine by iodine in aqueous media. J. biol. Chem. 96:285
    [Google Scholar]
  25. Singer T.P., Kearney E.B. 1954; Pathways of l-cysteine-sulphinate metabolism in animal tissues. Biochim. biophys.Acta 14:570
    [Google Scholar]
  26. Steinberg R.A. 1941; Sulphur and trace element nutrition of Aspergillus niger. J. agric. Res. 63:109
    [Google Scholar]
  27. Volkonsky M. 1933; Sur l’assimilation des sulfates per les Champignons: euthiotrophie et paratliiotrophie. C.R. Acad. Sci., Paris 197:712
    [Google Scholar]
  28. Westerman B.D., Rose W.C. 1928; The availability of disulphide acids as supplementing agents in diets deficient in cystine, II. α-hydroxy-β-dithiolpropionic acid. J. biol. Chem. 79:413
    [Google Scholar]
  29. Wohl A., Oesterlin C. 1901; Uberfiihrung der Weinsaure in Oxalessigsaure durch Wasserabspaltung bei niederer Temperatur. Ber. dtsch. chem. Ges. 34:1130
    [Google Scholar]
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