1887

Abstract

The extremely radioresistant bacterium encodes two genes that are homologous to those involved in bacterial lysine biosynthesis. In lysine biosynthesis, these genes are involved in the aminoadipate pathway and the diaminopimelate (DAP) pathway. is homologous to , which is essential for bacterial lysine biosynthesis via the aminoadipate pathway, and is homologous to , which is essential for lysine biosynthesis via the DAP pathway. In this study, and/or were disrupted. Each disruptant of and , and of or grew in a minimal medium, as did the wild-type. These results show that performs lysine biosynthesis in a unique way.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-148-9-2911
2002-09-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/148/9/1482911a.html?itemId=/content/journal/micro/10.1099/00221287-148-9-2911&mimeType=html&fmt=ahah

References

  1. Funayama T., Narumi I., Kikuchi M., Kitayama S., Watanabe H., Yamamoto K. 1999; Identification and disruption analysis of the recN gene in the extremely radioresistant bacterium Deinococcus radiodurans . Mutat Res 435:151–161 [CrossRef]
    [Google Scholar]
  2. Horowitz N. H. 1965; The evolution of biochemical syntheses – retrospect and prospect. In Evolving Genes and Proteins pp 16–23 Edited by Bryson V., Vogel H. J. New York: Academic Press;
    [Google Scholar]
  3. Jensen R. A. 1976; Enzyme recruitment in evolution of new function. Annu Rev Microbiol 30:409–425 [CrossRef]
    [Google Scholar]
  4. Kobashi N., Nishiyama M., Tanokura M. 1999; Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus : lysine is synthesized via α-aminoadipic acid not via diaminopimelic acid. J Bacteriol 181:1713–1718
    [Google Scholar]
  5. Kosuge T., Hoshino T. 1998; Lysine is synthesized through the α-aminoadipate pathway in Thermus thermophilus . FEMS Microbiol Lett 169:361–367
    [Google Scholar]
  6. Makarova K. S., Aravind L., Wolf Y. I., Tatusov R. L., Minton K. W., Koonin E. V., Daly M. J. 2001; Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol Mol Biol Rev 65:44–79 [CrossRef]
    [Google Scholar]
  7. Miyazaki J., Kobashi N., Nishiyama M., Yamane H. 2001; Functional and evolutionary relationship between arginine biosynthesis and prokaryotic lysine biosynthesis through α-aminoadipate. J Bacteriol 183:5067–5073 [CrossRef]
    [Google Scholar]
  8. Nishida H. 2001a; Distribution of genes for lysine biosynthesis through the aminoadipate pathway among prokaryotic genomes. Bioinformatics 17:189–191 [CrossRef]
    [Google Scholar]
  9. Nishida H. 2001b; Evolution of amino acid biosynthesis and enzymes with broad substrate specificity. Bioinformatics 17:1224–1225 [CrossRef]
    [Google Scholar]
  10. Nishida H., Nishiyama M. 2000; What is characteristic of fungal lysine synthesis through the α-aminoadipate pathway?. J Mol Evol 51:299–302
    [Google Scholar]
  11. Nishida H., Nishiyama M., Kobashi N., Kosuge T., Hoshino T., Yamane H. 1999; A prokaryotic gene cluster involved in synthesis of lysine through the amino adipate pathway: a key to the evolution of amino acid biosynthesis. Genome Res 9:1175–1183 [CrossRef]
    [Google Scholar]
  12. Parsot C., Saint-Girons I., Cohen G. N. 1987; Enzyme specialization during the evolution of amino acid biosynthetic pathways. Microbiol Sci 4:258–262
    [Google Scholar]
  13. Roy S. 1999; Multifunctional enzymes and evolution of biosynthetic pathways: retro-evolution by jumps. Proteins 37:303–309 [CrossRef]
    [Google Scholar]
  14. Schäfer S., Paalme T., Vilu R., Fuchs G. 1989; 13C-NMR study of acetate assimilation in Thermoproteus neutrophilus . Eur J Biochem 186:695–700 [CrossRef]
    [Google Scholar]
  15. Venkateswaran A., McFarlan S. C., Ghosal D., Minton K. W., Vasilenko A., Makarova K., Wackett L. P., Daly M. J. 2000; Physiologic determinants of radiation resistance in Deinococcus radiodurans . Appl Environ Microbiol 66:2620–2626 [CrossRef]
    [Google Scholar]
  16. White O., Eisen J. A., Heidelberg J. F. 29 other authors 1999; Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286:1571–1577 [CrossRef]
    [Google Scholar]
/content/journal/micro/10.1099/00221287-148-9-2911
Loading
/content/journal/micro/10.1099/00221287-148-9-2911
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error