1887

Abstract

The authors have characterized a chromosomally localized two-gene operon, , which encodes a putative P1-type ATPase, CueA, and a MerR-type metalloregulatory protein, CueR, in PNL-MK25. Disruption of by the insertion of mini-Tn:: into the wild-type strain led to a mutant strain with a sixfold reduction in its tolerance to copper; however, the tolerance of this mutant strain to the other seven related transition metals tested was not affected. The sensitivity of the mutant strain was attributed to a higher level of accumulation of intracellular copper, suggesting the involvement of CueA in copper export. Insertion of the cloned operon into the copper-sensitive mutant strain fully restored its tolerance to copper. :: expression studies confirmed that the operon was transcriptionally regulated by copper and CueR. Studies done on the mutant strain complemented with and revealed partial functional redundancy of and , respectively, in copper tolerance. Thus, the results of this study clearly suggest the involvement of in copper homeostasis in .

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2002-09-01
2020-04-09
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