1887

Abstract

The heterotrimeric G-protein α-subunit family plays multiple roles in eukaryotic cells, such as the regulation of growth and development, of pathogenicity and of the transmission of pheromone stimulation. In the homobasidiomycete , some genes encoding heterotrimeric G-protein α-subunits (α, α, , and ) have been reported. In this study, constitutively active mutants of , and were generated by site-directed mutagenesis and introduced into the monokaryon strain to investigate the function of each gene. Northern analysis showed that the mutated genes were strongly expressed when compared with endogenous G-proteins in many clones. Upon macroscopic examination, some transformed clones expressing (Q207R) and (Q204R) mutant genes exhibited a slight suppression of aerial-hyphae formation in the monokaryon strain. In contrast to the slight suppression of aerial-hyphae formation in the monokaryon, most clones expressing mutated or genes failed to form fruit-bodies in the dikaryon strain. This observation indicated that and played a role in suppressing fruit-body formation in the dikaryon. Furthermore, these phenotypes were similar to the phenotype of the mutant in to some extent. Since the gene encodes a putative regulator of the G-protein signalling protein (RGS), and might be targets of .

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2002-09-01
2020-04-02
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