RT Journal Article SR Electronic(1) A1 Mellits, Kenneth H. A1 Mullen, Joseph A1 Wand, Matthew A1 Armbruster, Gisèle A1 Patel, Amit A1 Connerton, Phillippa L. A1 Skelly, Maeve A1 Connerton, Ian F.YR 2002 T1 Activation of the transcription factor NF-κB by Campylobacter jejuni JF Microbiology, VO 148 IS 9 SP 2753 OP 2763 DO https://doi.org/10.1099/00221287-148-9-2753 PB Microbiology Society, SN 1465-2080, AB Campylobacter jejuni is a food-borne pathogen responsible for infectious enterocolitis. The early-response transcription factor NF-κB triggers the expression of genes associated with cellular immune and inflammatory responses. Co-incubation of HeLa cells with viable C. jejuni leads to the activation of the transcription factor NF-κB as determined by specific induction of a cellular luciferase-based reporter. Boiled cell-free extracts of C. jejuni are also potent dose-dependent stimulators of NF-κB-dependent transcription, the levels of which can reach up to 1000-fold as compared with independent controls. Using both cultured HeLa cells and human colonic epithelial (HCA-7) cells, the activation of NF-κB by C. jejuni boiled extract has been monitored through the degradation of IKBα and DNA binding of the nuclear translocated p50/p65 heterodimer of NF-κB. These events are co-ordinated with elaboration of the pro-inflammatory cytokine interleukin-8. Fractionation of the boiled C. jejuni extract suggests that the majority of the bioactive component has a molecular mass of 3 kDa or less, which is insensitive to proteinase K treatment., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-9-2753