1887

Abstract

Intracellular pathogens have developed different mechanisms which enable their survival and replication within the host cells. Some survive and replicate within a membrane-bound vacuole modified by the bacteria to support microbial growth (e.g. serovar Typhimurium), whereas others escape from the vacuole into the host cell cytosol, where they proliferate (e.g. ). In this study a strain carrying a mutation in which is released from the vacuole was used to analyse survival and replication within the cytosol of several cell lines. It was found that replicates within the cytosol of epithelial cells at a higher rate than that achieved when replicating within the vacuole, but is defective for replication in the cytosol of fibroblasts or macrophages. Using an double mutant strain which does not replicate within host cells, it was shown that encounters a killing activity within the cytosol of macrophages. Furthermore, experiments using cytosol extracted from either infected or uninfected macrophages suggested that this activity is activated upon infection.

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2002-09-01
2020-04-09
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