1887

Abstract

The cAMP signal transduction pathway controls many processes in fungi. The gene, encoding the regulatory subunit (PKA-R) of cAMP-dependent protein kinase (PKA), was cloned from the industrially important filamentous fungus . To investigate the involvement of PKA in morphology of , a set of transformants which overexpressed or (encoding the catalytic subunit of PKA) either individually or simultaneously was prepared as well as mutants in which and/or were disrupted. Strains overexpressing or both and could not be distinguished from the wild-type, suggesting that regulation of PKA activity is normal in these strains. Absence of PKA activity resulted in a two- to threefold reduction in colony diameter on plates. The most severe phenotype was observed in the absence of PKA-R, i.e., very small colonies on plates, absence of sporulation and complete loss of growth polarity during submerged growth. Suppressor mutations easily developed in the Δ mutant and one of these mutants appeared to lack PKA-C activity. These data suggest that cAMP-dependent protein phosphorylation in regulates growth polarity and formation of conidiospores.

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2002-08-01
2022-01-23
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