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Volume 148, Issue 8

Protective role of trehalose during severe oxidative stress caused by hydrogen peroxide and the adaptive oxidative stress response in Free

Abstract

The cellular response to the oxidative stress caused by hydrogen peroxide and its putative correlation with the stress protector trehalose was investigated in CAI.4 and the / double mutant, which is deficient in trehalose synthesis. When exponential wild-type blastoconidia were exposed to high concentrations of hydrogen peroxide, they displayed a high cell survival, accompanied by a marked rise of intracellular trehalose. The latter is due to a moderate activation of trehalose synthase and the concomitant inactivation of neutral trehalase. Identical challenge in the / double mutant severely reduced cell viability, a phenotype which was suppressed by overexpression of the gene. Pretreatment of growing cultures from both strains with either a low, non-lethal concentration of HO (05 mM) or a preincubation at 37 °C, induced an adaptive response that protected cells from being killed by a subsequent exposure to oxidative stress. During these mild oxidative preincubations, trehalose was not induced in CAI.4 cells and remained undetectable in their / counterpart. Blastoconidia from the two strains exhibited a similar degree of cell protection during the adaptive response. The induction of trehalose accumulation by HO was not due to an increased expression of mRNA. These results are consistent with a mainly protective role of trehalose in during direct oxidative stress but not during acquired oxidative tolerance.

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Keyword(s): adaptive response , cell protector , opportunistic yeast pathogen , oxidative stress , ROS, reactive oxygen species , T-6P synthase, trehalose-6-phosphate synthase and TPS1
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2002-08-01
2024-12-07
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Protective role of trehalose during severe oxidative stress caused by hydrogen peroxide and the adaptive oxidative stress response in Candida albicans
Microbiology 148, 2599 (2002); https://doi.org/10.1099/00221287-148-8-2599
/content/journal/micro/10.1099/00221287-148-8-2599
/content/journal/micro/10.1099/00221287-148-8-2599
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