1887

Microbiology

Volume 148, Issue 8

Identification and characterization of novel small RNAs in the – intergenic region of the genome Free

Abstract

A novel RNA species was isolated from , and its sequence was determined and mapped to its genetic position. This RNA was termed BS190 RNA from the length of its mature form (190 nt), and the gene encoding it is located within the – intergenic region of the genome. Northern blotting revealed that the novel RNA species is transcribed in vegetative cells as a larger precursor (BS201 RNA, 201 nt). After transcription, the 5′ end of the precursor is processed to generate the mature form, BS190 RNA. A computer-aided prediction of the secondary structure of BS190 RNA showed that it can be folded into a single hairpin structure with some bulge structures. The authors found that the growth rate of a ΔBS190 mutant strain of was reduced when compared to the wild-type. A phylogenetic comparison of the sequence of the BS190 RNA gene with sequences from the databases suggests that RNA related to BS190 RNA appears to be encoded in the genomes of and

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): non-coding RNA , RNA processing , RNA secondary structure , scRNA, small cytoplasmic RNA , sRNA, small RNA and tmRNA, transfer-message RNA
© Microbiology Society
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2002-08-01
2021-10-19
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Identification and characterization of novel small RNAs in the aspS–yrvM intergenic region of the Bacillus subtilis genome
Microbiology 148, 2591 (2002); https://doi.org/10.1099/00221287-148-8-2591
/content/journal/micro/10.1099/00221287-148-8-2591
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