1887

Abstract

Silencing of the operon is mediated by histone-like protein H-NS and affected by other pleiotropic regulators, including sigma factor RpoS. Silencing is relieved and the operon is activated in mutants and by mutations that map in the vicinity of the promoter. However, the expression level of activated operon derivatives varies with the strain background. Here it is shown that the repression of the operon by RpoS requires Crl. Crl is a protein that is necessary for the RpoS-dependent expression of the operon and that enhances the expression of other RpoS-dependent genes. In a Crl-negative strain RpoS had no effect on the operon. The gene maps close to the locus in the operon region and is deleted in many commonly used strains. Crl may therefore account for some of the observed strain-dependent variations of operon expression levels and effects of pleiotropic regulators on operon regulation.

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2002-08-01
2019-10-21
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