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Volume 148, Issue 8

Evolution of multi-gene segments in the intergenic region of serovar Typhimurium LT2

aThe GenBank accession number for the sequence reported in this paper is AY050714.

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Abstract

The nucleotide sequence of the 12·6 kb region between the and genes of serovar Typhimurium LT2 () was compared to other enteric bacterial intergenic regions. The – region is composed of three distinct segments, designated HK, O and S, as defined by sequence similarities to contiguous ORFs in other bacteria. Inverted chromosomal orientations of each of these segments are found between the and genes in related . The HK segment is distantly related to a cluster of seven ORFs found in and a cluster of five ORFs found between the and genes in K-12. The O segment is related to the intergenic region found in O157:H7 and type 1. The third segment, S, is common to diverse species, but is absent from . Despite the extensive collinearity and conservation of the overall genetic maps of and K-12, the insertions, deletions and inversions in the region provide evidence that this region of the chromosome is an active site for horizontal gene transfer and rearrangement.

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Keyword(s): EHEC, enterohaemorrhagic Escherichia coli , EPEC, enteropathogenic Escherichia coli , Escherichia coli , horizontal gene transfer , mutS , rpoS , S. typhi, Salmonella enterica serovar Typhi , S. typhimurium, Salmonella enterica serovar Typhimurium and SARC, Salmonella Reference Collection C
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2002-08-01
2024-04-23
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References

  1. Andre A., Puca A., Sansone F., Brandi A., Antico G., Calogero R. A. 2000; Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis -elements unrelated to canonical translation initiation signals. FEBS Lett 468:73–78 [CrossRef]
     [Google Scholar]
  2. Blattner F. R., Plunkett G. 3rd, Bloch C. A. 14 other authors 1997; The complete genome sequence of Escherichia coli K-12. Science 277:1453–1474 [CrossRef]
     [Google Scholar]
  3. Boyd E. F., Wang F. S., Whittam T. S., Selander R. K. 1996; Molecular genetic relationships of the salmonellae. Appl Environ Microbiol 62:804–808
     [Google Scholar]
  4. Brown E. W., LeClerc J. E., Li B., Payne W. L., Cebula T. A. 2001a; Phylogenetic evidence for horizontal transfer of mutS alleles among naturally occurring Escherichia coli strains. J Bacteriol 183:1631–1644 [CrossRef]
     [Google Scholar]
  5. Brown E. W., LeClerc J. E., Kotewicz M. L., Cebula T. A. 2001b; Three R’s of bacterial evolution: how replication, repair, and recombination frame the origin of species. Environ Mol Mutagen 38:248–260 [CrossRef]
     [Google Scholar]
  6. Brown E. W., Kotewicz M. L., Cebula T. A. 2002; Detection of recombination in Salmonella enterica using the incongruence length difference test. Mol Phylogenet Evol 24:102–120 [CrossRef]
     [Google Scholar]
  7. Carter P. E., Butler L., Booth I. R., Thomson-Carter F. M. 1999; Characterization of the mutS–rpoS region from STEC and non-STEC. In Abstracts of the 99th General Meeting of the American Society for Microbiology p 237 Washington, DC: American Society for Microbiology;
     [Google Scholar]
  8. Culham D. E., Wood J. M. 2000; An Escherichia coli reference collection group B2- and uropathogen-associated polymorphism in the rpoS–mutS region of the E. coli chromosome. J Bacteriol 182:6272–6276 [CrossRef]
     [Google Scholar]
  9. Dauga C. 2002; Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae : a model molecule for molecular systematic studies. Int J Syst Evol Microbiol 52:531–547
     [Google Scholar]
  10. Denamur E., Lecointre G., Darlu P. 9 other authors 2000; Evolutionary implications of the frequent horizontal transfer of mismatch repair genes. Cell 103:711–721 [CrossRef]
     [Google Scholar]
  11. de Rosa R., Labedan B. 1998; The evolutionary relationships between the two bacteria Escherichia coli and Haemophilus influenzae and their putative last common ancestor. Mol Biol Evol 15:17–27 [CrossRef]
     [Google Scholar]
  12. Eisen J. A., Heidelber J. F., White O., Salzberg S. L. 2000; Evidence for symmetric chromosomal inversions around the replication origin in bacteria. Genome Biol 1:RESEARCH0011.1–0011.9
     [Google Scholar]
  13. Hacker J., Kaper J. B. 2000; Pathogenicity islands and the evolution of microbes. Annu Rev Microbiol 54:641–679 [CrossRef]
     [Google Scholar]
  14. Hartman P. E., Hartman Z., Stahl R. C. 1971; Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella . Adv Genet 16:1–34
     [Google Scholar]
  15. Herbelin C. J., Chirillo S. C., Melnick K. A., Whittam T. S. 2000; Gene conservation and loss in the mutS–rpoS genomic region of pathogenic Escherichia coli . J Bacteriol 182:5381–5390 [CrossRef]
     [Google Scholar]
  16. Hughes D. 2000; Evaluating genome dynamics: the constraints on rearrangements within bacterial genomes. Genome Biol 1: REVIEWS0006.1–0006.8
    [Google Scholar]
  17. Kupchella E., Koch W. H., Cebula T. A. 1994; Mutant alleles of tRNA ( Thr ) genes suppress the hisG46 missense mutation in Salmonella typhimurium. Environ Mol Mutagen. 2381–88 [CrossRef]
  18. Lawrence J. G., Roth J. R. 1996; Selfish operons: horizontal transfer may drive the evolution of gene clusters. Genetics 143:1843–1860
     [Google Scholar]
  19. LeClerc J. E., Cebula T. A. 2000; Pseudomonas survival strategies in cystic fibrosis. Science 289:391–392
     [Google Scholar]
  20. LeClerc J. E., Li B., Payne W. L., Cebula T. A. 1996; High mutation frequencies among Escherichia coli and Salmonella pathogens. Science 274:1208–1211 [CrossRef]
     [Google Scholar]
  21. LeClerc J. E., Li B., Payne W. L., Cebula T. A. 1999; Promiscuous origin of a chimeric sequence in the Escherichia coli O157: H7 genome. J Bacteriol 181:7614–7617
     [Google Scholar]
  22. Liu S. L., Sanderson K. E. 1996; Highly plastic chromosomal organization in Salmonella typhi . Proc Natl Acad Sci USA 93:10303–10308 [CrossRef]
     [Google Scholar]
  23. Liu S. L., Sanderson K. E. 1998; Homologous recombination between rrn operons rearranges the chromosome in host-specialized species of Salmonella . FEMS Microbiol Lett 164:275–281 [CrossRef]
     [Google Scholar]
  24. Liu S. L., Hessel A., Sanderson K. E. 1993; Genomic mapping with I-Ceu I, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli , and other bacteria. Proc Natl Acad Sci USA 90:6874–6878 [CrossRef]
     [Google Scholar]
  25. Liu G. R., Rahn A., Liu W. Q., Sanderson K. E., Johnston R. N., Liu S. L. 2002; The evolving genome of Salmonella enterica serovar Pullorum. J Bacteriol 184:2626–2633 [CrossRef]
     [Google Scholar]
  26. Martin K., Morlin G., Smith A., Nordyke A., Eisenstark A., Golomb M. 1998; The tryptophanase gene cluster of Haemophilus influenzae type b: evidence for horizontal gene transfer. J Bacteriol 180:107–118
     [Google Scholar]
  27. Matic I., Rayssiguier C., Radman M. 1995; Interspecies gene exchange in bacteria: the role of SOS and mismatch repair systems in evolution of species. Cell 80:507–515 [CrossRef]
     [Google Scholar]
  28. McClelland M., Sanderson K. E., Spieth J. 23 other authors 2001; Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 413:852–856 [CrossRef]
     [Google Scholar]
  29. Mills D. M., Bajaj V., Lee C. A. 1995; A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion genes is absent from the corresponding region of the Escherichia coli K-12 chromosome. Mol Microbiol 15:749–759
     [Google Scholar]
  30. Missiakas D., Raina S. 1997; Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli : role of two new phosphoprotein phosphatases PrpA and PrpB. EMBO J 16:1670–1685 [CrossRef]
     [Google Scholar]
  31. Neidhardt F. C., Curtiss R. III, Ingraham J. L. 7 other editors 1996 Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn. vols I and II Washington, DC: American Society for Microbiology;
     [Google Scholar]
  32. Pancetti A., Galan J. E. 2001; Characterization of the mutS -proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes. FEMS Microbiol Lett 197:203–208 [CrossRef]
     [Google Scholar]
  33. Parkhill J., Dougan G., James K. D. 38 other authors 2001; Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature 413:848–852 [CrossRef]
     [Google Scholar]
  34. Perez-Rueda E., Collado-Vides J. 2001; Common history at the origin of the position–function correlation in transcriptional regulators in archaea and bacteria. J Mol Evol 53:172–179 [CrossRef]
     [Google Scholar]
  35. Reeves M. W., Evins G. M., Heiba A. A., Plikaytis B. D., Farmer J. J. 3rd (1989; Clonal nature of Salmonella typhi and its genetic relatedness to other salmonellae as shown by multilocus enzyme electrophoresis and proposal of Salmonella bongori comb. nov. J Clin Microbiol 27:313–320
     [Google Scholar]
  36. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  37. Sanderson K. E., Hessel A., Shu-Lin L., Rudd K. E. others 1996; The genetic map of Salmonella typhimurium , Edition VIII. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn. pp 1903–1999 Edited by Neidhardt F. C. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  38. Shi L., Kehres D. G., Maguire M. E. 2001; The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties. J Bacteriol 183:7053–7057 [CrossRef]
     [Google Scholar]
  39. Smith C. M., Koch W. H., Franklin S. B., Foster P. L., Cebula T. A., Eisenstadt E. 1990; Sequence analysis and mapping of the Salmonella typhimurium LT2 umuDC operon. J Bacteriol 172:964–978
     [Google Scholar]
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Evolution of multi-gene segments in the mutS–rpoS intergenic region of Salmonella enterica serovar Typhimurium LT2aaThe GenBank accession number for the sequence reported in this paper is AY050714.
Microbiology 148, 2531 (2002); https://doi.org/10.1099/00221287-148-8-2531
/content/journal/micro/10.1099/00221287-148-8-2531
/content/journal/micro/10.1099/00221287-148-8-2531
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