1887

Abstract

The entomopathogen secretes many proteins during the late stages of insect larvae infection and during laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of . PrtA is secreted via a classical type I secretory pathway and is encoded within the operon . The 405 bp gene encodes a 148 kDa pre-protein that is translocated to the periplasm by the classical signal-peptide-dependent pathway, yielding the mature 119 kDa inhibitor Inh. Inh is a specific inhibitor of the protease PrtA. This study describes the purification of Inh and the initial characterization of its protease inhibition properties.

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2002-08-01
2020-03-29
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