1887

Abstract

Vanadium has an antibacterial activity against , especially under conditions of iron limitation. Some degree of resistance to V is inducible by prior exposure to the metal. One mutant (VS1) with a higher sensitivity to V was obtained by transposon mutagenesis of PA 59.20, a clinical isolate. This mutant had an insertion in a non-coding region, upstream of a cluster of four genes. Three of them show similarities to genes corresponding to known antibiotic efflux systems, including an efflux protein, a membrane fusion protein and an outer-membrane porin. This cluster was named -. By allelic exchange, three mutants, (for on-oding egion), and were constructed in PAO1. Next to V sensitivity, the , and mutants also showed reduced production of elastase, rhamnolipids, pyocyanine, pyoverdine and had reduced swarming motility, phenotypes that are known to be regulated by quorum sensing. All wild-type phenotypes, including growth in the presence of V, were restored by complementation with the complete cluster. The production of -acyl-homoserine lactones (AHLs) was detected using the bioassay. Total extracts from the three mutants failed to induce the production of violacein by , although AHLs were detected by TLC and overlay. Violacein production was restored by complementation with -. The mutant grew very slowly in LB or CAA medium, indicating that OpmD has an important physiological function for the cell. In conclusion, it is believed that the MexGHI-OpmD pump is probably involved in AHL homeostasis in

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2002-08-01
2020-08-07
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