1887

Abstract

A gene encoding sorbitol-6-phosphate dehydrogenase (SorF) belonging to the sorbose operon () has been characterized in . Inactivation of this gene revealed the presence of another sorbitol-6-phosphate dehydrogenase that was induced by D-sorbitol (D-glucitol). The gene encoding this activity () has also been isolated, sequenced and disrupted. The sorbitol-6-phosphate dehydrogenase genes (, ) were required for growth on L-sorbose and D-sorbitol, respectively. Biochemical and transcriptional analyses of the wild-type and mutant strains demonstrated that L-sorbose and D-sorbitol induced and the gene encoding the sorbose operon activator (), while the expression of was only activated by D-sorbitol. Furthermore, these studies indirectly suggested that a common metabolite of the L-sorbose and D-sorbitol metabolic pathways (probably D-sorbitol 6-phosphate) would act as the effector of SorR. The same effector would also be the inducer of , although the two pathways seem to be subject to distinct regulatory mechanisms.

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2002-08-01
2020-01-28
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