@article{mbs:/content/journal/micro/10.1099/00221287-148-8-2293, author = "Dong, Z. and Zelmer, C. D. and Canny, M. J. and McCully, M. E. and Luit, B. and Pan, B. and Faustino, R. S. and Pierce, G. N. and Vessey, J. K.", title = "Evidence for protection of nitrogenase from O2 by colony structure in the aerobic diazotroph Gluconacetobacter diazotrophicus", journal= "Microbiology", year = "2002", volume = "148", number = "8", pages = "2293-2298", doi = "https://doi.org/10.1099/00221287-148-8-2293", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-8-2293", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "diffusion resistance", keywords = "N2 fixation", keywords = "CLSM, confocal laser scanning microscopy", keywords = "nitrogen", keywords = "Acetobacter diazotrophicus", keywords = "oxygen", keywords = "DAI, days after inoculation", keywords = "pO2, partial pressure of oxygen", abstract = " Gluconacetobacter diazotrophicus is an endophytic diazotroph of sugarcane which exhibits nitrogenase activity when growing in colonies on solid media. Nitrogenase activity of G. diazotrophicus colonies can adapt to changes in atmospheric partial pressure of oxygen (pO2). This paper investigates whether colony structure and the position of G. diazotrophicus cells in the colonies are components of the bacterium’s ability to maintain nitrogenase activity at a variety of atmospheric pO2 values. Colonies of G. diazotrophicus were grown on solid medium at atmospheric pO2 of 2 and 20 kPa. Imaging of live, intact colonies by confocal laser scanning microscopy and of fixed, sectioned colonies by light microscopy revealed that at 2 kPa O2 the uppermost bacteria in the colony were very near the upper surface of the colony, while the uppermost bacteria of colonies cultured at 20 kPa O2 were positioned deeper in the mucilaginous matrix of the colony. Disruption of colony structure by physical manipulation or due to ‘slumping’ associated with colony development resulted in significant declines in nitrogenase activity. These results support the hypothesis that G. diazotrophicus utilizes the path-length of colony mucilage between the atmosphere and the bacteria to achieve a flux of O2 that maintains aerobic respiration while not inhibiting nitrogenase activity.", }