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Volume 148, Issue 7

The multigene family for the ATP-dependent Clp protease in the cyanobacterium

cThe GenBank accession numbers for the sequences of - and - reported in this paper are U92039 and AJ132005, respectively.

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Abstract

In the cyanobacterium sp. strain PCC 7942 a multigene family of three different isozymes encodes the proteolytic subunit ClpP of the ATP-dependent Clp protease. In contrast to the monocistronic gene, and are part of two bicistronic operons with and , respectively. Unlike most bacterial Clp proteins, the ClpP2, ClpP3, ClpR and ClpX proteins were not highly inducible by high temperatures, or by other stresses such as cold, high light or oxidation, although slower gradual rises occurred for all four proteins during high light, and for ClpP3, ClpR and ClpX at low temperature. Attempts to inactivate the , , or genes were only successful for , suggesting the others are essential for cell viability. The Δ mutant exhibited no significant phenotypic changes from the wild-type, including no change in ClpX content. Despite the apparent bicistronic arrangement of both - and -, all four genes primarily produce monocistronic transcripts, although polycistronic transcripts were detected. Mapping of 5′ ends for the and monocistronic transcripts revealed promoters situated within the 3′ region of and , respectively. Transcriptional and translational studies further showed differences in the expression and regulation between the -- genes. Inactivation of caused a significant decrease in ClpP2 protein concomitant to small increases in both ClpP3 and ClpR. Inactivation of resulted in a large rise in transcripts but to a lesser extent in ClpP1 protein. Similar small increases in ClpP3, ClpR and ClpX proteins also occurred in Δ. These results highlight the regulatory complexity of these multiple genes and their functional importance in cyanobacteria.

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Keyword(s): Chl, chlorophyll , cyanobacteria , gene expression , MBP, maltose-binding protein , protein regulation , proteolysis and stress
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2002-07-01
2024-04-19
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The clpP multigene family for the ATP-dependent Clp protease in the cyanobacterium SynechococcusccThe GenBank accession numbers for the sequences of clpPII-clpX and clpR-clpPIII reported in this paper are U92039 and AJ132005, respectively.
Microbiology 148, 2255 (2002); https://doi.org/10.1099/00221287-148-7-2255
/content/journal/micro/10.1099/00221287-148-7-2255
/content/journal/micro/10.1099/00221287-148-7-2255
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