1887

Abstract

A previously undescribed haemolysin, distinct from the major O1 El Tor haemolysin, HlyA, was cloned from the O1 classical biotype strain Z17561. This novel haemolysin showed 715% overall similarity to the δ-thermostable direct haemolysin of , and so it has been termed δ-thermostable haemolysin (Vc-δTH, encoded by the gene). An ORF found immediately downstream, which appears to be transcriptionally and translationally linked to , displayed strong homology to the family of acyl-CoA synthetases. When expressed from an inducible promoter in , Vc-δTH was shown to be a 228 kDa protein active on sheep red blood cells. Co-expression of with had no effect on the haemolytic activity or cytoplasmic localization of Vc-δTH. A Z17561 ::Km mutant showed unaltered behaviour in the infant mouse cholera model.

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2002-07-01
2019-10-23
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