1887

Abstract

Two pectin lyase genes, designated and , were cloned from f. sp. , a pathogen of round-leaved mallow (). was isolated using cDNA from infected plant material; was isolated using cDNA from 3-day-old mycelia grown in mallow-cell-wall extract (MCWE) broth. is the first pectinase gene described thus far to encode a cellulose-binding domain (CBD), which is common in cellulases and xylanases, whereas encodes a pectin lyase that lacks a CBD. In pure culture, expression could be detected when purified pectin or glucose was the sole carbon source, but not when MCWE was the sole carbon source. The lack of expression appeared to be due to gene repression by some unknown factor(s) in the cell-wall extract. In contrast, expression of was detected in cultures when MCWE, but not when purified pectin or glucose, was the sole carbon source. In infected tissue, detection of expression by Northern-blot hybridization and by RT-PCR began with the onset of the necrotrophic phase of infection. Expression of was not detectable by Northern-blot hybridization, but was observed byRT-PCR in both the biotrophic and necrotrophic phases of infection. The differences between and (i.e. encoding a CBD and differences in the expression patterns of both genes) may be related to the requirements of f. sp. to be able to grow in host tissue under the different conditions present during the biotrophic and necrotrophic phases of infection.

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2002-07-01
2020-01-27
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