1887

Abstract

569 (ATCC 10876) endospores germinate in response to inosine or L-alanine, the most rapid germination response being elicited by a combination of these germinants. The operon has already been characterized as a homologue of the spore-germination receptor family of operons found in all spp. examined; the primary defect in mutant spores is in the inosine germination response, although spores were also slower to germinate in L-alanine. Additional transposon-insertion mutants, from similar Tn-LTV1 mutagenesis and enrichment experiments, now define two more operons, also members of the family of homologues, important in L-alanine and inosine germination. Transposon insertions were identified in an alanine-specific germination locus, named , which represents an operon of three genes, termed , and By examining the residual germination response to L-alanine in and mutants, it was deduced that the GerL proteins contribute most strongly to the L-alanine germination response, and that the GerI proteins, required primarily in inosine germination, mediate only much slower germination responses to alanine. The L-alanine germination responses mediated by GerL and GerI proteins differ in their germination rates, temperature optima and germinant concentration dependence. The locus, again identified by transposon insertion, is a second inosine-related germinant-receptor operon. GerQ and GerI proteins are both required for the germination response to inosine as sole germinant, but GerQ has no role in L-alanine germination. Although near-identical homologues of and operons are evident in the genome sequence, there is no evidence of a close homologue of

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2002-07-01
2020-02-25
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