1887

Abstract

The Mig protein of is a type III immunoglobulin G (IgG)-binding protein, expressing IgG- and α-macroglobulin (α-M)-binding receptors. This study showed that the Mig protein also displays binding activities to bovine immunoglobulin A (B-IgA). Biotin-labelled bovine serum IgA bound immobilized recombinant Mig and α-M receptors derived from Mig, as well as the native Mig extracted from the surface of . strain SDG8 and the α-M receptor released from the isogenic mutant strain Mig8-Mt, as determined by Western blotting and ELISA. There was no B-IgA binding activity to the immobilized IgG receptor derived from Mig or the proteins in the culture supernatant from the mutant strain Mig7-Mt, in which expression of Mig or Mig-related peptides on the cell surface was completely abolished. In a reciprocal experiment, biotin-labelled Mig was found to bind immobilized bovine serum IgA but not human IgA (H-IgA). The binding of Mig to bovine serum IgA was competitively inhibited by unlabelled Mig, intact and truncated α-M receptors, and bovine serum IgA, but not by the Mig-IgG receptor, H-IgA or B-IgG. The binding of Mig and partially purified bovine secretory IgA (B-sIgA) was also characterized by Western blotting. Membrane-immobilized B-sIgA did not react with the biotin-labelled Mig, whereas soluble B-sIgA showed binding activity to the immobilized α-M receptor of Mig. It is therefore concluded that the 11 kDa N-terminal region of the α-M receptor of the Mig protein specifically binds soluble and immobilized bovine serum IgA, as well as soluble B-sIgA. This is believed to be the first report of a B-IgA-binding protein in

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2002-07-01
2020-03-28
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