1887

Abstract

When a shotgun phage-display library of Newman was affinity selected (panned) against recombinant von Willebrand factor (vWf), a novel von Willebrand factor binding protein (vWbp) was found. Experimental data indicate that the interaction between vWbp and vWf is very specific and mediated by a region of 26 aa residues in the C-terminal part of vWbp. vWbp has an N-terminal secretory signal sequence but no cell wall anchoring motif, suggesting a soluble extracellular location. Mature vWbp could be purified from the culture supernatant and the identity of the protein was confirmed by N-terminal sequencing. vWbp migrates with an apparent molecular mass of 66 kDa and the deduced protein consists of 482 aa. The gene encoding vWbp, named , was present in all strains investigated.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-148-7-2037
2002-07-01
2020-08-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/148/7/1482037a.html?itemId=/content/journal/micro/10.1099/00221287-148-7-2037&mimeType=html&fmt=ahah

References

  1. Coulter S. N., Schwan W. R., Ng E. Y.. 7 other authors 1998; Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments. Mol Microbiol30:393–404[CrossRef]
    [Google Scholar]
  2. Darouiche R. O., Landon G. C., Patti J. M., Nguyen L. L., Fernau R. C., McDevitt D., Greene C., Foster T., Klima M. 1997; Role of Staphylococcus aureus surface adhesins in orthopaedic device infections: are results model-dependent?. J Med Microbiol46:75–79[CrossRef]
    [Google Scholar]
  3. Fearon D. T., Austin K. F. 1975; Properdin: binding to C3b and stabilisation of the C3b-dependent C3 convertase. J Exp Med142:856–863[CrossRef]
    [Google Scholar]
  4. Fischer B. E., Schlokat U., Reiter M., Mundt W., Dorner F. 1997; Biochemical and functional characterization of recombinant von Willebrand factor produced on a large scale. Cell Mol Life Sci53:943–950[CrossRef]
    [Google Scholar]
  5. Fujigaki Y., Yousif Y., Morioka T., Batsford S., Vogt A., Hishida A., Miyasaka M. 1998; Glomerular injury induced by cationic 70-kD staphylococcal protein; specific immune response is not involved in early phase in rats. J Pathol184:436–445[CrossRef]
    [Google Scholar]
  6. Gemmell C. G., Goutcher S. C., Reid R., Sturrock R. D. 1997; Role of certain virulence factors in a murine model of Staphylococcus aureus arthritis. J Med Microbiol46:208–213[CrossRef]
    [Google Scholar]
  7. Hacker J., Blum-Oehler G., Muhldorfer I., Tschape H. 1997; Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution. Mol Microbiol23:1089–1097[CrossRef]
    [Google Scholar]
  8. Hartleib J., Kohler N., Dickinson R. B.. 7 other authors 2000; Protein A is the von Willebrand factor binding protein on Staphylococcus aureus . Blood96:2149–2156
    [Google Scholar]
  9. Hienz S. A., Schennings T., Heimdahl A., Flock J. I. 1996; Collagen binding of Staphylococcus aureus is a virulence factor in experimental endocarditis. J Infect Dis174:83–88[CrossRef]
    [Google Scholar]
  10. Hussein M., Becker K., von Eiff C., Schrenzel J., Peters G., Herrmann M. 2001; Identification and characterization of a novel 38·5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma protein. J Bacteriol183:6778–6786[CrossRef]
    [Google Scholar]
  11. Ikeda Y., Handa M., Kawano K.. 9 other authors 1991; The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress. J Clin Invest87:1234–1240[CrossRef]
    [Google Scholar]
  12. Jacobsson K., Frykberg L. 1995; Cloning of ligand-binding domains of bacterial receptors by phage display. Biotechniques18:878–885
    [Google Scholar]
  13. Jacobsson K., Frykberg L. 1996; Phage display shot-gun cloning of ligand-binding domains of prokaryotic receptors approaches 100% correct clones. Biotechniques20:1070–1076
    [Google Scholar]
  14. Jacobsson K., Frykberg F. 1999; Gene VIII-based, phage display vectors for selection against complex mixtures of ligands. In Expression Genetics: Accelerated and High-throughput Methods pp225–238 Edited by McClelland M., Pardee A. B.. Natick, MA: Eaton Publishing;
    [Google Scholar]
  15. Jönsson K., McDevitt D., McGavin M. H., Patti J. M., Hook M. 1995; Staphylococcus aureus expresses a major histocompatibility complex class II analog. J Biol Chem270:21457–21460[CrossRef]
    [Google Scholar]
  16. Kuroda M., Ohta T., Uchiyama I.. 34 other authors 2001; Whole genome sequencing of meticillin-resistant Staphylococcus aureus . Lancet357:1225–1240[CrossRef]
    [Google Scholar]
  17. Lawler J., Hynes R. O. 1986; The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins. J Cell Biol103:1635–1648[CrossRef]
    [Google Scholar]
  18. Lindmark R., Movitz J., Sjoquist J. 1977; Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus . Eur J Biochem74:623–628[CrossRef]
    [Google Scholar]
  19. Lowy F. D. 1998; Staphylococcus aureus infections. N Engl J Med339:520–532[CrossRef]
    [Google Scholar]
  20. McDevitt D., François P., Vaudaux P., Foster T. J. 1994; Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus . Mol Microbiol11:237–248[CrossRef]
    [Google Scholar]
  21. McGavin M. H., Krajewska-Pietrasik D., Ryden C., Hook M. 1993; Identification of a Staphylococcus aureus extracellular matrix-binding protein with broad specificity. Infect Immun61:2479–2485
    [Google Scholar]
  22. Mohlke K. L., Nichols W. C., Ginsburg D. 1999; The molecular basis of von Willebrand disease. Int J Clin Lab Res29:1–7[CrossRef]
    [Google Scholar]
  23. Moreillon P., Entenza J. M., Francioli P., McDevitt D., Foster T. J., François P., Vaudaux P. 1995; Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis. Infect Immun63:4738–4743
    [Google Scholar]
  24. Ni Eidhin D., Perkins S., François P., Vaudaux P., Hook M., Foster T. J. 1998; Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus . Mol Microbiol30:245–257[CrossRef]
    [Google Scholar]
  25. Nolan K. F., Kalutz S., Higgins J. M., Goundis D., Reid K. B. 1992; Characterization of the human properdin gene. Biochem J287:291–297
    [Google Scholar]
  26. Palma M., Nozohoor S., Schennings T., Heimdahl A., Flock J. I. 1996; Lack of the extracellular 19-kilodalton fibrinogen-binding protein from Staphylococcus aureus decreases virulence in experimental wound infection. Infect Immun64:5284–5289
    [Google Scholar]
  27. Palma M., Haggar A., Flock J. I. 1999; Adherence of Staphylococcus aureus is enhanced by an endogenous secreted protein with broad binding activity. J Bacteriol181:2840–2845
    [Google Scholar]
  28. Patel A. H., Nowlan P., Weavers E. D., Foster T. 1987; Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele replacement. Infect Immun55:3103–3110
    [Google Scholar]
  29. Patti J. M., Bremell T., Krajewska-Pietrasik D., Abdelnour A., Tarkowski A., Ryden C., Hook M. 1994; The Staphylococcus aureus collagen adhesin is a virulence determinant in experimental septic arthritis. Infect Immun62:152–161
    [Google Scholar]
  30. Rhem M. N., Lech E. M., Patti J. M., McDevitt D., Hook M., Jones D. B., Wilhelmus K. R. 2000; The collagen-binding adhesin is a virulence factor in Staphylococcus aureus keratitis. Infect Immun68:3776–3779[CrossRef]
    [Google Scholar]
  31. Ruggeri Z. M. 1999; Structure and function of von Willebrand factor. Thromb Haemostasis82:576–584
    [Google Scholar]
  32. Ruggeri Z. M., Ware J. 1993; von Willebrand factor. FASEB J7:308–316
    [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  34. Savage B., Saldivar E., Ruggeri Z. M. 1996; Initiation of platelet adhesion by arrest onto fibrinogen or translocation on von Willebrand factor. Cell84:289–297[CrossRef]
    [Google Scholar]
  35. Smeltzer M. S. 2000; Characterization of staphylococcal adhesins for adherence to host tissues. In Handbook of Bacterial Adhesion. Principles, Methods, and Applications pp411–444 Edited by An Y. H., Friedman R. J.. Totowa, NJ: Humana Press;
    [Google Scholar]
  36. Wann E. R., Fehringer A. P., Ezepchuk Y. V., Schlievert P. M., Bina P., Reiser R. F., Hook M. M., Leung D. Y. 1999; Staphylococcus aureus isolates from patients with Kawasaki disease express high levels of protein A. Infect Immun67:4737–4743
    [Google Scholar]
  37. Weiss H. J., Hawiger J., Ruggeri Z. M., Turitto V. T., Thiagarajan P., Hoffmann T. 1989; Fibrinogen-independent platelet adhesion and thrombus formation on subendothelium mediated by glycoprotein IIb–IIIa complex at high shear rate. J Clin Invest83:288–297[CrossRef]
    [Google Scholar]
  38. Wilm M., Shevchenko A., Houthaeve T., Breit S., Schweigerer L., Fotsis T., Mann M. 1996; Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature378:466–469
    [Google Scholar]
  39. Wolz C., McDevitt D., Foster T. J., Cheung A. L. 1996; Influence of agr on fibrinogen binding in Staphylococcus aureus Newman. Infect Immun64:3142–3147
    [Google Scholar]
  40. Xie L., Chesterman C. N., Hogg P. J. 2001; Control of von Willebrand factor multimer size by thrombospondin-1. J Exp Med193:1341–1349[CrossRef]
    [Google Scholar]
  41. Yatohgo T., Izumi M., Kashiwagi H., Hayashi M. 1988; Novel purification of vitronectin from human plasma by heparin affinity chromatography. Cell Struct Funct13:281–292[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-148-7-2037
Loading
/content/journal/micro/10.1099/00221287-148-7-2037
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error