1887

Abstract

The gene encoding the cystathionine β-lyase (CBL) of subsp. NCDO 1489 was cloned and expressed in . Overexpression of CBL complemented the methionine auxotrophy of an mutant, demonstrating that this enzyme functions as a CBL. However, PatC is distinguishable from the MetC CBLs by a low identity in amino acid sequence, a sensitivity to iodoacetic acid, greater thermostability and a lower substrate affinity. Homologues of were detected in the 13 strains studied, but only seven of them showed CBL activity. In constrast to CBL strains, all CBL-deficient strains analysed were auxotrophic for methionine. This supports the hypothesis that CBLs from lactobacilli are probably involved in methionine biosynthesis. Moreover, the results of this study suggest that post-transcriptional mechanisms account for the differences in CBL activities observed between strains of .

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2002-07-01
2020-01-24
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