Two human faeces carriage isolates of Listeria monocytogenes (H1 and H2) were compared to reference strains (ScottA and LO28) with regard to their lethality in 14-day-old chick embryos, their haemolytic and phospholipase (phosphatidylcholine-phospholipase C and phosphatidylinositol-phospholipase C) activities and their invasiveness towards Caco-2 cells. Experimental infection of chick embryos allowed discrimination of the strains into those exhibiting high virulence (ScottA and H2), those exhibiting slightly attenuated virulence (LO28) and those exhibiting low virulence (H1). A similar percentage mortality and time to death for embryos was observed when they were infected with H2 as was seen with infection by the reference strain ScottA. Therefore, human carriage strain H2 was considered potentially pathogenic. In contrast to H2 and ScottA, H1 exhibited low virulence. Using the tissue-culture cell-line model, it was found that carriage strain H1 was unable to enter Caco-2 cells efficiently, even though it was similar to the virulent strains in terms of the enzymic activities involved in pathogenicity. Detection of the internalins InlA and InlB, involved in the internalization of L. monocytogenes in the host cells, by immunoblot indicated that a truncated form of InlA was produced by H1. Taken together, these data provide a starting point for the study of the behaviour of two types of human faeces carriage strains and their characterization.
BarbourA. H.,
RamplingA.,
HormaecheC. E.
2001; Variation in the infectivity of Listeria monocytogenes isolates following intragastric inoculation of mice. Infect Immun 69:4657–4660[CrossRef]
BraunL.,
DramsiS.,
DehouxP.,
BierneH.,
LindahlG.,
CossartP.
1997; InlB: an invasion protein of Listeria monocytogenes with a novel type of surface association. Mol Microbiol 25:285–294[CrossRef]
BraunL.,
NatoF.,
PayrastreB.,
MazieJ. C.,
CossartP.
1999; The 213-amino-acid leucine-rich repeat region of the Listeria monocytogenes InlB protein is sufficient for entry into mammalian cells, stimulation of PI 3-kinase and membrane ruffling. Mol Microbiol 34:10–23[CrossRef]
BroschR.,
CatimelB.,
MilonG.,
BuchrieserE.,
VindelE.,
RocourtJ.
1993; Virulence heterogeneity of Listeria monocytogenes strains from various sources (food, human, animal) in immunocompetent mice and its association with typing characteristics. J Food Protect 56:296–301
BuncicS.,
AveryS. M.
1996; Relationship between variations in pathogenicity and lag phase at 37 °C of Listeria monocytogenes previously stored at 4 °C. Lett Appl Microbiol 23:18–22[CrossRef]
ChakrabortyT.,
EbelF.,
WehlandJ.,
DufrenneJ.,
NotermansS.
1994; Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes. FEMS Immunol Med Microbiol 10:1–9[CrossRef]
CoffeyA.,
RomboutsF. M.,
AbeeT.
1996; Influence of environmental parameters on phosphatidylcholine phospholipase C production in Listeria monocytogenes : a convenient method to differentiate L. monocytogenes from other Listeria species. Appl Environ Microbiol 62:1252–1256
CotterP. D.,
GahanC. G. M.,
HillC.
2001; A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluids. Mol Microbiol 40:465–475[CrossRef]
Del CorralF.,
BuchananR. L.,
BencivengoM. M.,
CookeP. H.
1990; Quantitative comparison of selected virulence associated characteristics in food and clinical isolates of Listeria
. J Food Protect 53:1003–1009
DramsiS.,
BiswasI.,
MaguinE.,
BraunL.,
MastroeniP.,
CossartP.
1995; Entry of Listeria monocytogenes into hepatocytes requires expression of inlB , a surface protein of the internalin multigene family. Mol Microbiol 16:251–261[CrossRef]
DurstJ.,
ZimanyiM.
1976; Listeria monocytogenes – trägertum von an geburtshilflichen arbeitlungen tätigen in epidemiefreien perioden. Zentbl Bakteriol (Orig A 234:281–283
FujisawaT.,
MoriM.
1994; Evaluation of media for determining hemolytic activity and that of API Listeria system for identifying strains of Listeria monocytogenes
. J Clin Microbiol 32:1127–1129
GaillardJ. L.,
BercheP.,
MounierJ.,
RichardS.,
SansonettiP.
1987; In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun 55:2822–2829
GaillardJ. L.,
BercheP.,
FrehelC.,
GouinE.,
CossartP.
1991; Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell 65:1127–1141[CrossRef]
GouletV.,
LepoutreA.,
RocourtJ.,
CourtieuP.,
DehaumontP.,
VeitP.
1993; Epidémie de listériose en France. Bilan final et résultats de l’enquête épidémiologique. Bull Epidémiol Hebdom 4:13–14
JonquièresR.,
BierneH.,
MengaudJ.,
CossartP.
1998; The inlA gene of Listeria monocytogenes LO28 harbors a nonsense mutation resulting in release of internalin. Infect Immun 66:3420–3422
KampelmacherE. H.,
van Noorle JansenL. M.
1972; Über die isolierung von L. monocytogenes bei klinisch gesunden personen. Zentbl Bakteriol (Orig A) 221:70–77
LecuitM.,
OhayonH.,
BraunL.,
MengaudJ.,
CossartP.
1997; Internalin of Listeria monocytogenes with an intact leucine-rich repeat region is sufficient to promote internalization. Infect Immun 65:5309–5319
LecuitM.,
DramsiS.,
GottardiC.,
Fedor-ChaikenM.,
GumbinerB.,
CossartP.
1999; A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes
. EMBO J 18:3956–3963[CrossRef]
ManoharM.,
BaumannD. O.,
BosN. A.,
CebraJ. J.
2001; Gut colonization of mice with actA -negative mutant of Listeria monocytogenes can stimulate a humoral mucosal immune response. Infect Immun 69:3542–3549[CrossRef]
MengaudJ.,
LecuitM.,
LebrunM.,
NatoF.,
MazieJ. C.,
CossartP.
1996a; Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin. Infect Immun 64:5430–5433
MengaudJ.,
OhayonH.,
GounonP.,
MegeR. M.,
CossartP.
1996b; E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells. Cell 84:923–932[CrossRef]
NorrungB.,
AndersenJ. K.
2000; Variations in virulence between different electrophoretic types of Listeria monocytogenes
. Lett Appl Microbiol 30:228–232[CrossRef]
NotermansS. H.,
DufrenneJ.,
Leimeister-WachterM.,
DomannE.,
ChakrabortyT.
1991; Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species. Appl Environ Microbiol 57:2666–2670
RoussetA.,
Lemaı̃treJ. P.,
DelcourtA.
1994; Recherche de Listeria monocytogenes au niveau de différents sites (selles, sécrétions génitales et rhino-pharyngés). Med Mal Infect1174–1179
SantiagoN. I.,
ZipfA.,
BhuniaA. K.
1999; Influence of temperature and growth phase on expression of a 104-kilodalton Listeria adhesion protein in Listeria monocytogenes
. Appl Environ Microbiol 65:2765–2769
SchlechW. F.III.
1984; New perspectives on the gastrointestinal mode of transmission in invasive Listeria monocytogenes infection. Clin Invest Med 7:321–324
SchlechW. F.III,
LavigneP. M.,
BortolussiR. A.8 other authors1983; Epidemic listeriosis – evidence for transmission by food. N Engl J Med 308:203–206[CrossRef]
SchuchatA.,
DeaverK.,
HayesP. S.,
GravesL.,
MascolaL.,
WengerJ. D.
1993; Gastrointestinal carriage of Listeria monocytogenes in household contacts of patients with listeriosis. J Infect Dis 167:1261–1262[CrossRef]
TerplanG.,
SteinmeyerS.
1989; Investigations on the pathogenicity of Listeria spp. by experimental infection of the chick embryo. Int J Food Microbiol 8:277–280[CrossRef]
WiedmannM.,
BruceJ. L.,
KeatingC.,
JohnsonA. E.,
McDonoughP. L.,
BattC. A.
1997; Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect Immun 65:2707–2716
ZacharZ.,
SavageD. C.
1979; Microbial interference and colonization of the murine gastrointestinal tract by Listeria monocytogenes
. Infect Immun 23:168–174
Assessment of the pathogenic potential of two Listeria monocytogenes human faecal carriage isolatesThe GenBank accession number for the sequence reported in this paper is AF468816.