1887

Abstract

The RecA protein is one of the best-studied enzymes, but less is understood about how RecA homologues of other species are similar to or different from the RecA. In the Gram-negative pathogen (the gonococcus; Gc), the causative agent of gonorrhoea, RecA is involved in DNA transformation, pilin antigenic variation, and DNA repair. By expressing the genes from Gc and under control of regulatory sequences in , the authors have shown that the Gc RecA fully complements an mutant for homologous recombination, but only partially complements for survival to DNA damage. By expressing similar constructs in Gc, it was shown that the RecA complements for pilin antigenic variation, partially complements for DNA transformation, but does not complement for survival to DNA damage, suggesting that species-specific interactions are important for DNA repair, but not for homologous recombination. Co-expression of the and genes in Gc suggests that in this heterologous system RecX modulates RecA-mediated processes.

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2002-06-01
2019-10-20
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