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Microbiology Society logo Microbiology

Volume 148, Issue 6

A third fatty acid Δ9-desaturase from with a different substrate specificity to ole1p and ole2p

The EMBL accession number for the sequence reported in this paper is AJ278339.

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Abstract

A third gene (Δ9-3) encoding a fatty acid Δ9-desaturase was isolated from the oil-producing fungus . The predicted protein of 512 aa shared 53% sequence identity with the two fatty acid Δ9-desaturases, ole1p and ole2p, already described in this organism and contained three histidine boxes, four putative transmembrane domains and a C-terminal cytochrome fusion that are typical of most fungal membrane-bound fatty acid desaturases. However, unlike the and genes, the Δ9-3 ORF failed to complement the mutation. GC-MS analysis of fatty-acid-supplemented yeast transformants containing the Δ9-3 gene indicated that this enzyme had negligible activity with endogenous palmitic acid (16:0) as substrate and moderate activity (30–65% desaturation) with endogenous stearic acid (18:0). Yeast transformants overexpressing any one of the three fatty acid Δ9-desaturase genes or the gene produced low amounts of hexacosenoic acid [26:1(n-9)], a fatty acid that is not normally present in yeast cells. It follows that these Δ9-desaturases may also display low n-9 desaturation activity with very long-chain saturated fatty acid substrates. Conversely, high levels of desaturase in the endoplasmic reticulum membrane of these yeast transformants may increase the availability of suitable monounsaturated substrates for fatty acid elongation.

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Keyword(s): 16:0, palmitic acid , 16:1, palmitoleic acid , 18:0, stearic acid , 18:1, oleic acid , 18:2, linoleic acid , 18:3, α-linolenic acid , 20:0, arachidic acid , 20:1, eicosenoic acid , 20:3, dihomo-γ-linolenic acid , 20:4, arachidonic acid , 22:0, behenic acid , 22:1, erucic acid , 24:0, lignoceric acid , 24:1, nervonic acid , 26:0, hexacosanoic acid , 26:1, hexacosenoic acid , DMOX, 4,4-dimethyloxazoline , ELO2 and ELO3, genes encoding fatty acid elongases , ER, endoplasmic reticulum , FAME, fatty acid methyl ester , fatty acid elongation , fatty acid n-9 desaturation , hexacosenoic acid , LCPUFA, long-chain polyunsaturated fatty acid , ole1 and ole2, genes encoding fatty acid Δ9-desaturases ole1p and ole2p, respectively , RACE, rapid amplification of cDNA ends , stearoyl-CoA desaturase , UTR, untranslated region of mRNA , yeast complementation , γ-18:3, γ-linolenic acid and Δ9-3, gene encoding fatty acid Δ9-desaturase homologue
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2002-06-01
2025-04-30
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/content/journal/micro/10.1099/00221287-148-6-1725
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A third fatty acid Δ9-desaturase from Mortierella alpina with a different substrate specificity to ole1p and ole2pThe EMBL accession number for the sequence reported in this paper is AJ278339.
Microbiology 148, 1725 (2002); https://doi.org/10.1099/00221287-148-6-1725
/content/journal/micro/10.1099/00221287-148-6-1725
/content/journal/micro/10.1099/00221287-148-6-1725
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